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master's thesis
Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2021. urn:nbn:hr:178:669266
Čelebić, Inga
University of Zagreb
Faculty of Veterinary Medicine

Institutional repository: Repository of Faculty of Veterinary Medicine

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Čelebić, I. (2021). Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:669266

Čelebić, Inga. "Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:178:669266

Čelebić, Inga. "Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:178:669266

Čelebić, I. (2021). 'Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 05 September 2024, https://urn.nsk.hr/urn:nbn:hr:178:669266

Čelebić I. Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2021 [cited 2024 September 05] Available at: https://urn.nsk.hr/urn:nbn:hr:178:669266

I. Čelebić, "Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:178:669266

Metadata
TitlePraćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši
Title (english)Monitoring of specific antibody titers induced by autogenous vaccine against APEC strains of E. coli in broiler parent flocks
AuthorInga Čelebić
MentorŽeljko Gottstein (mentor)
Committee memberDanijela Horvatek Tomić (predsjednik povjerenstva)
Committee memberŽeljko Gottstein (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
Zagreb
Defense date and country2021-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Public Health and Food Safety
Abstract
APEC strains (Avian pathogenic Escherichia coli) are the main cause of collibacilosis in poultry. They inflict major economic damage by causing high morbidity and mortality in chicks, broilers and laying hens. Also, chicken meat products contaminated with APEC present danger for human health. Appearance of resistant and multiresistant strains as a result of long term, excessive use of antibiotics, has widely limited attainability of effective antimicrobial substances and forced us to search for more effective options for reducing the incidence of collibacilosis and large economical loses caused by it. Production and administration of autogenous vaccines is a very effective method in disease prevention. In this research, microbiological monitoring of the delivered carcasses enabled isolation of E. coli strains in the broiler parent flock farms. After that, typing of the isolates was conducted and an autogenous vaccine was produced for vaccination of pullets and roosters at 9 and 19 weeks of age. For determining specific antibody titre, an ELISA test was made from inactivated antigen used for manufacturing of the vaccine. Results proved a significant titre increase after application of the autogenous vaccine. Application of aluminium hydroxide as adjuvant stimulated fast rise of the titre, but also a constant drop of titre. Oil adjuvant on the other hand stimulates long lasting titre which is double in values in vaccinated flocks compared to unvaccinated older flocks.
Abstract (english)
APEC strains (Avian pathogenic Escherichia coli) are the main cause of collibacilosis in poultry. They inflict major economic damage by causing high morbidity and mortality in chicks, broilers and laying hens. Also, chicken meat products contaminated with APEC present danger for human health. Appearance of resistant and multiresistant strains as a result of long term, excessive use of antibiotics, has widely limited attainability of effective antimicrobial substances and forced us to search for more effective options for reducing the incidence of collibacilosis and large economical loses caused by it. Production and administration of autogenous vaccines is a very effective method in disease prevention. In this research, microbiological monitoring of the delivered carcasses enabled isolation of E. coli strains in the broiler parent flock farms. After that, typing of the isolates was conducted and an autogenous vaccine was produced for vaccination of pullets and roosters at 9 and 19 weeks of age. For determining specific antibody titre, an ELISA test was made from inactivated antigen used for manufacturing of the vaccine. Results proved a significant titre increase after application of the autogenous vaccine. Application of aluminium hydroxide as adjuvant stimulated fast rise of the titre, but also a constant drop of titre. Oil adjuvant on the other hand stimulates long lasting titre which is double in values in vaccinated flocks compared to unvaccinated older flocks.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:669266
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-12-01 12:39:25