The activity of wild animals depends on different external and internal factors, among which the most important are light and temperature, gender and reproductive status, prey or predator activity, and human activity. This research aimed to follow the seasonal changes in the activity of the Eurasian lynx in Croatia and determine if lynx activity on microlocations changes during the year. The research has been conducted on the area of the lynx range in Croatia, where 77 camera traps have been set on different microlocations in the period of the 4th of May 2018 to the 7th of December 2021. During the beforementioned period, there have been noted 927 events of lynx, of which the largest number of events (11.8%) has been noted in February when the mating season starts. The largest number of those events in February have been males on marking sites. Although during the beforementioned period, a similar amount of camera traps have been set up on marking sites, forrest roads, and animal paths, the number of events captured on marking sites has been at least two times bigger. Furthermore, there is a clear difference in the usage of wildlife trails and forest roads during the year: from January to May lynx use wildlife trails more often whereas from June to December they use forest roads more frequently.