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master's thesis
GENOTIPIZACIJA POPULACIJE KONJA U TIPU DALMATINSKOG BUŠAKA
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2022. urn:nbn:hr:178:053486
Listeš, Ema
University of Zagreb
Faculty of Veterinary Medicine

Institutional repository: Repository of Faculty of Veterinary Medicine

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Listeš, E. (2022). GENOTIPIZACIJA POPULACIJE KONJA U TIPU DALMATINSKOG BUŠAKA (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:053486

Listeš, Ema. "GENOTIPIZACIJA POPULACIJE KONJA U TIPU DALMATINSKOG BUŠAKA." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:178:053486

Listeš, Ema. "GENOTIPIZACIJA POPULACIJE KONJA U TIPU DALMATINSKOG BUŠAKA." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:178:053486

Listeš, E. (2022). 'GENOTIPIZACIJA POPULACIJE KONJA U TIPU DALMATINSKOG BUŠAKA', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:178:053486

Listeš E. GENOTIPIZACIJA POPULACIJE KONJA U TIPU DALMATINSKOG BUŠAKA [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2022 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:178:053486

E. Listeš, "GENOTIPIZACIJA POPULACIJE KONJA U TIPU DALMATINSKOG BUŠAKA", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:178:053486

Metadata
TitleGENOTIPIZACIJA POPULACIJE KONJA U TIPU DALMATINSKOG BUŠAKA
Title (english)GENOTYPING OF DALMATIAN BUŠAK HORSE TYPE POPULATION The
AuthorEma Listeš
MentorMaja Maurić Maljković (mentor)
MentorNikica Prvanović Babić (mentor)
Committee memberSven Menčik (predsjednik povjerenstva)
Committee memberMaja Maurić Maljković (član povjerenstva)
Committee memberNikica Prvanović Babić (član povjerenstva)
Committee memberAnamaria Ekert Kabalin (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
Zagreb
Defense date and country2022-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Animal Production and Biotechnology
Abstract
Sve je veća opasnost izumiranja rijetkih lokalnih populacija, a zajedno s njima i njihovog lokalno prilagođenog genetskog fonda. U očuvanju biološke raznolikosti domaćih životinja bitan se naglasak stavlja na očuvanje i sprječavanje izumiranja autohtonih pasmina životinja, posebice lokalnih izvornih pasmina poput dalmatinskog bušaka. Danas je dalmatinski bušak svrstan u nedovoljno poznate odnosno neocijenjene pasmine. Na području Dalmacije nalazimo malu populaciju konja koja morfološkim karakteristikama odgovara konjima u tipu bušaka. Istraživanje je provedeno na 38 jedinki konja ( 11 mužjaka i 27 ženki). Istraživanoj populaciji uzete su mjere i uspoređene su sa pronađenim podacima iz povijesti te sa postojećom literaturom. Molekularno-genetičkim metodama određena je zastupljenost pojedinih mikrosatelitnih lokusa i haplogrupa mtDNK pasmine. Mjereni konji su vanjštinom, uz vrlo mala odstupanja, odgovarali vanjštini konja iz povijesnih zapisa te iz prethodnih istraživanja, a izrazito su manjeg tjelesnog okvira od ostalih hrvatskih autohtonih pasmina konja. Svi analizirani mikrosatelitni lokusi bili su svi polimorfni. Prosječna opažena heterozigotnost (HO) iznosi 74%, očekivana (HE) 76% te posljedično tome fiksacijski indeks je približno jednak nuli (FIS=0,02). Prosječni informacijski sadržaj polimorfizma (PIC), kao i za većinu lokusa zasebno, ukazuje na visoko informativne markere. Prema analizama mtDNK, kod konja u tipu dalmatinskog bušaka nalazimo 10 različitih haplotipova koji su raspodijeljeni u 8 glavnih grupa (A, E, G, L, M, O, P i Q).
Abstract (english)
The growing concern of extinction of rare local populations, and with them their locally adapted genetic fund. In preserving the biodiversity of domestic animals, significant acent is placed on the preservation and prevention of extinction of indigenous animal breeds, especially local native breeds such as the Dalmatian bušak. Today, the Dalmatian bušak is classified as an insufficiently known or not evaluated breed. In Dalmatia there is a small population of horses whose morphological characteristics correspond to horses in the type of dalamtian bušak. This study was conducted on 38 horses (11 males and 27 females). The studied horses were measured to compare them to histrocal and literature data. Molecular genetic methods were used to determine the prevalence of individual microsatellite loci and haplogroups of the mtDNA. The exterior of all measured horses, with very small deviations, corresponded to the appearance of horses from historical and literature data. Also, they were distinctively smaller than other Croatian autochthonous horse breeds. All investigated microsatellite loci were polymorphic. The mean observed heterozygosity (HO) was 74%, expected (HE) 76% and, consequently, the fixation index was approximately zero (FIS = 0.02). The mean information content of polymorphisms (PIC) indicated highly informative markers. According to mtDNA analyzes, in horses of the Dalmatian bušak type we found 10 different haplotypes that were divided into 8 main groups (A, E, G, L, M, O, P and Q).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:053486
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-04-20 09:01:47