prikaz prve stranice dokumenta Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama)
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master's thesis
Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama)
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2014. urn:nbn:hr:178:455970
Vranešević, Ines
University of Zagreb
Faculty of Veterinary Medicine
Department of Biology

Institutional repository: Repository of Faculty of Veterinary Medicine

Cite this document

Vranešević, I. (2014). Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama) (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:455970

Vranešević, Ines. "Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama)." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:178:455970

Vranešević, Ines. "Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama)." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:178:455970

Vranešević, I. (2014). 'Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama)', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 12 July 2024, https://urn.nsk.hr/urn:nbn:hr:178:455970

Vranešević I. Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2014 [cited 2024 July 12] Available at: https://urn.nsk.hr/urn:nbn:hr:178:455970

I. Vranešević, "Kreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama)", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:178:455970

Metadata
TitleKreiranje vrsno specifičnih početnica za umnažanje mitohondrijske DNA jelena lopatara (Dama dama)
Title (english)Design of Species Specific Primers for Amplification of Fallow Deer (Dama dama) Mitochondrial DNA
AuthorInes Vranešević
MentorTomislav Gomerčić (mentor)
MentorMagda Sindičić (mentor)
Committee memberDean Konjević
Committee memberMaja Popović
Committee memberTomislav Gomerčić
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
(Department of Biology)
Zagreb
Defense date and country2014-12-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Basic and Preclinical Veterinary Sciences
Abstract
Ciljevi ovog rada bili su dizajnirati vrsno specificne pocetnice za umnažanje kontrolne regije mitohondrijske DNA jelena lopatara (Dama dama), te optimizirati PCR protokol za korištenje tih pocetnica. Pocetnice su dizajnirane temeljem sekvenci jelena lopatara preuzetih iz genske baze podataka GenBank uz korištenje WEB aplikacije za izradu pocetnica. Odredena je duljina pocetnica, mjesto vezivanja i duljina PCR produkta. Pocetnice se koriste za identifikaciju jelena lopatara i razlikovanje od ostalih vrsta, koristeci samo izolaciju DNA, PCR i elektroforezu. Upotreba pocetnica ispitana je na osam uzoraka jelena lopatara, tri uzorka jelena obicnog i tri uzorka srne.Tako dizajnirane vrsno specificne pocetnice moguce je primjenjivati u ekološkim istraživanjima, istraživanjima prehrane predatora, u forenzickim slucajevima i u kontroli mesnih namirnica u prehrambenoj industriji.
Abstract (english)
Goals of this work were to design species specific primers for amplification of control region of mitochondrial DNA for fallow deer (Dama dama) and to find optimal PCR protocol for application of those primers. Primers have been designed based on fallow deer sequences from GenBank using the WEB application for primer design. I have defined the size, location of and the size of the PCR product. With these specific primers fallow deer can be identified and distinguished from the other animal species using only DNA isolation, PCR and electrophoresis. Application of the primers has been tested on eight samples of fallow deer, three samples of roe deer and three reed deer samples. These species specific primers can be applied in ecological researches, researches of predators feeding habits, in forensic cases and in control of game meat products in food industry.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:455970
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-01-18 09:14:55