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master's thesis
PRIMJENA MULTIPLEKS LANČANE REAKCIJE POLIMERAZOM ZA DOKAZIVANJE TCDA I TCDB GENA ZA TOKSINE BAKTERIJE CLOSTRIDIOIDES DIFFICILE U FECESU PASA
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2022. urn:nbn:hr:178:636900
Kamber, Magda
University of Zagreb
Faculty of Veterinary Medicine

Institutional repository: Repository of Faculty of Veterinary Medicine

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Kamber, M. (2022). PRIMJENA MULTIPLEKS LANČANE REAKCIJE POLIMERAZOM ZA DOKAZIVANJE TCDA I TCDB GENA ZA TOKSINE BAKTERIJE CLOSTRIDIOIDES DIFFICILE U FECESU PASA (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:636900

Kamber, Magda. "PRIMJENA MULTIPLEKS LANČANE REAKCIJE POLIMERAZOM ZA DOKAZIVANJE TCDA I TCDB GENA ZA TOKSINE BAKTERIJE CLOSTRIDIOIDES DIFFICILE U FECESU PASA." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:178:636900

Kamber, Magda. "PRIMJENA MULTIPLEKS LANČANE REAKCIJE POLIMERAZOM ZA DOKAZIVANJE TCDA I TCDB GENA ZA TOKSINE BAKTERIJE CLOSTRIDIOIDES DIFFICILE U FECESU PASA." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:178:636900

Kamber, M. (2022). 'PRIMJENA MULTIPLEKS LANČANE REAKCIJE POLIMERAZOM ZA DOKAZIVANJE TCDA I TCDB GENA ZA TOKSINE BAKTERIJE CLOSTRIDIOIDES DIFFICILE U FECESU PASA', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 18 November 2024, https://urn.nsk.hr/urn:nbn:hr:178:636900

Kamber M. PRIMJENA MULTIPLEKS LANČANE REAKCIJE POLIMERAZOM ZA DOKAZIVANJE TCDA I TCDB GENA ZA TOKSINE BAKTERIJE CLOSTRIDIOIDES DIFFICILE U FECESU PASA [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2022 [cited 2024 November 18] Available at: https://urn.nsk.hr/urn:nbn:hr:178:636900

M. Kamber, "PRIMJENA MULTIPLEKS LANČANE REAKCIJE POLIMERAZOM ZA DOKAZIVANJE TCDA I TCDB GENA ZA TOKSINE BAKTERIJE CLOSTRIDIOIDES DIFFICILE U FECESU PASA", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:178:636900

Metadata
TitlePRIMJENA MULTIPLEKS LANČANE REAKCIJE POLIMERAZOM ZA DOKAZIVANJE TCDA I TCDB GENA ZA TOKSINE BAKTERIJE CLOSTRIDIOIDES DIFFICILE U FECESU PASA
Title (english)Performance of multiplex polymerase chain reaction for detection of tcdA and tcdB genes of Clostridioides difficile toxins in feces of dogs
AuthorMagda Kamber
MentorSuzana Hađina (mentor)
Committee memberJosipa Habuš (predsjednik povjerenstva)
Committee memberZrinka Štritof (član povjerenstva)
Committee memberSuzana Hađina (član povjerenstva)
Committee memberVilim Starešina (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
Zagreb
Defense date and country2022-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Veterinary Public Health and Food Safety
Abstract
Clostridioides difficile je gram-pozitivna, anaerobna, štapićasta, sporulirajuća bakterija koja proizvodi glavna dva toksina: toksin A i toksin B čija je sinteza kodirana tcdA odnosno tcdB genom. Smatra se da toksogeni sojevi dovode do nastanka različitih kliničkih znakova bolesti probavnog sustava, od blagih samoograničavajućih proljeva pa sve do teških oblika hemoragičnih gastroenteritisa. Cilj ovog istraživanja bio je utvrditi prisutnost gena tcdA i tcdB za toksine bakterije Clostridioides difficile u fecesu zdravih pasa i pasa s kliničkim znakovima bolesti probavnog sustava koristeći multipleks lančanu reakciju polimerazom te njihovu ulogu u nastanku probavnih poremećaja u pasa. U ovom istraživanju analizirano je 116 uzoraka fecesa pasa, odnosno 49 uzoraka fecesa zdravih pasa te 67 uzoraka fecesa pasa s kliničkim znakovima bolesti probavnog sustava. U 5,2% uzoraka utvrđena je prisutnost tpi gena bakterije C. difficile, od kojih je u 2,6% utvrđen toksogeni soj (A+B-), a u njih 2,6% netoksogeni soj (A-B-). U zdravih životinja utvrđena je prisutnost tpi gena, odnosno netoksogeni soj bakterije C. difficile (A-B-). U tri psa s kliničkim znakovima bolesti probavnog sustava utvrđena je prisutnost tcdA gena za toksin A, odnosno toksogeni soj (A+B-), što ukazuje na moguću povezanost toksogenih sojeva s nastankom kliničkih znakova bolesti probavnog sustava, dok je u jednog psa iz te skupine utvrđen netoksogeni soj (A-B-). Rezultati ovog istraživanja ukazuju na potrebu daljnjeg rasvjetljavanja prisutnosti i uloge bakterije C. difficile u nastanku bolesti probavnog sustava u pasa.
Abstract (english)
Clostridioides difficile is a gram-positive, anaerobic, rod-shaped, sporulating bacterium that produces two main types of toxins: toxin A and toxin B whose production is encoded by the tcdA and tcdB genes. Toxigenic strains are thought to lead to the occurrence of various gastrointestinal symptoms, ranging from mild self-limiting diarrhea to severe forms of hemorrhagic gastroenteritis. The aim of this study was to determine the presence of the tcdA and tcdB genes for C. difficile toxins in the feces of healthy dogs and dogs with symptoms of gastrointestinal disease using multiplex polymerase chain reaction, and to determine their role in the development of digestive disorders in dogs. In this study, 116 dog fecal samples were analyzed, i.e., 49 fecal samples from healthy dogs and 67 fecal samples from dogs with gastrointestinal symptoms. The tpi gene was detected in 5.2% of the fecal samples, with the toxigenic strain (A+B-) detected in 2.6%, and the non-toxigenic strain (A-B-) detected in 2.6% of the samples. In healthy animals, only the tpi gene was detected, i.e., a non-toxigenic strain of C. difficile (A-B-). In three dogs with symptoms of gastrointestinal disease the tcdA gene of toxin A was detected, i.e., a toxigenic strain (A+B+), indicating a possible association between toxigenic strains and the occurrence of gastrointestinal symptoms, while the sample of one dog from this group was identified as a non-toxigenic strain (A-B-). The results of this study indicate that further research is needed on the presence and role of C. difficile in the occurrence of gastrointestinal disease in dogs.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:178:636900
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-09-12 11:18:03