Shift work is a way of organizing the work of employees under the employer's established working hours, which is done during the morning, afternoon or night of day, or in the first, second or third shift. The above method of work organization brings many benefits to the employer, to the company and to the society which expects that goods and services are available 24 hours a day. However, that way of work has shortcomings and negative impact on the health of workers: effect on changes in mental and physical state workers, slowly undermines their health, and family and interpersonal relationships. Analysis of the impact of shift work, its advantages and disadvantages to employees is the purpose of this final work. For the purposes of this analysis, data from various secondary sources, and the survey was conducted with 30 employees in Zagreb working in shifts at night to check workers' opinion on shift and night work and analyzed the impact of shift and night work on their psychophysical and health status. In the study in this paper, conducted on a small sample of employees of the company in Zagreb, whose work is organized through the shift work, it has been confirmed numerous world′s studies about the harmful effects of shift work on the mental and physical state of workers.