The aim of this study was to determine the concentration of mrcury in ten wild edible mushrooms and the substrate, to assess the role of individual species as biological indicators of environmental pollution. In this study we analyzed five saprophytic species (Agaricus campestris (L) Fries, Armilariella mellea (Vahl. ex Fr.) Karst., Clitocybe inversa (Scop. ex Fr.) Pat., Clitocybe nebularis Batsch. ex Fr., Macrolepiota procera (Scop. ex Fr.) Sing.,) and five ectomycorrhizal species (Boletus aestivalis Paulet ex Fries., Boletus edulis Bull ex Fries., Lactarius deterrimus Groger., Tricholoma portentosum (Fr.) Quelet, Tricholoma terreum (Schff. ex Fr.) Kummer). The concentration of mercury in a solution adapted to read the individual element was determined by measuring absorption at the atomic absorption spectrometer AAS (SOLAR-THERMO SCIENTIFIC, type M5 AA System) according to HRN ISO 11466: 2004. Significant differences were found in the concentrations of mercury between analysed saprophytic and ectomycorrhizal species of mushrooms (p<0,05). The highest mean concentrations of mercury was determined in Boletus aestivalis species in area Island Krk (2,01 mg kg-1) and Labinstina (2,02 mg kg-1). The average distribution rates of mercury inanatomical parts of the fruiting body were different. Significantly higher concentration of mercury was determined in the cap in relation to stipe of analyzed mushrooms (p<0,001). Bio-accumulation features were established in all of the investigated mushroom species (BCF>1), whereby the best bio-indicators of environmental pollution of mercury mushrooms from the genus Boletus. By comparing the concentration of mercury in mushrooms and the legal act, it can be concluded that consuming them does not have a negative impact on human health.