prikaz prve stranice dokumenta Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije
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master's thesis
Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije
Zagreb: University of Zagreb, Faculty of Agriculture, 2019. urn:nbn:hr:204:778601
Levanić, Marko
University of Zagreb
Faculty of Agriculture
Fisheries, Beekeeping, Game Management and Special Zoology

Institutional repository: Repository Faculty of Agriculture University of Zagreb

Cite this document

Levanić, M. (2019). Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:778601

Levanić, Marko. "Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije." Master's thesis, University of Zagreb, Faculty of Agriculture, 2019. https://urn.nsk.hr/urn:nbn:hr:204:778601

Levanić, Marko. "Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije." Master's thesis, University of Zagreb, Faculty of Agriculture, 2019. https://urn.nsk.hr/urn:nbn:hr:204:778601

Levanić, M. (2019). 'Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 20 December 2024, https://urn.nsk.hr/urn:nbn:hr:204:778601

Levanić M. Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2019 [cited 2024 December 20] Available at: https://urn.nsk.hr/urn:nbn:hr:204:778601

M. Levanić, "Kemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:204:778601

Metadata
TitleKemijska karakterizacija pčelinjeg otrova primjenom infracrvene (FTIR-ATR) spektroskopije
Title (english)Chemical characterization of honey bee venom using infrared (FTIR-ATR) spectroscopy
AuthorMarko Levanić
MentorLidija Svečnjak (mentor)
Committee memberLidija Svečnjak (predsjednik povjerenstva)
Committee memberDragan Bubalo (član povjerenstva)
Committee memberMarko Vinceković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Fisheries, Beekeeping, Game Management and Special Zoology)
Zagreb
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Pčelinji je otrov (apitoksin) vrijedan proizvod medonosne pčele (Apis melifera L.) sa širokom primjenom i rastućom potraţnjom u farmaceutskoj i kozmetičkoj industriji, Glavninu njegova sastava čine peptidi i enzimi (60-70%). Cilj je ovog rada bio provesti detaljnu kemijsku karakterizaciju otrova medonosne pčele metodom infracrvene spektroskopije. Uzorci pčelinjeg otrova prikupljeni su iz 3 pokusne pčelinje zajednice izravno iz ţalca pčela radilica različite dobi i metodom elektrostimulacije pčela (skupni uzorak) te analiziran metodom FTIR-ATR spektroskopije. Rezultati su pokazali kako pčelinji otrov daje karakterističan FTIR-ATR spektralni profil u kojem dominiraju apsorpcijske vrpce proteina (enzima) i peptida. Rezultati spektralne analize pčelinjeg otrova pčela radilica različite dobi (7, 14, 24 i 42 dana) pokazali su kako je kvalitativni i kvantitativni sastav otrova postojan tijekom cijelog ţivota pčele, dok je analiza skupnog uzorka otrova prikupljenog iz pokusnih zajednica ukazala na kontaminaciju prikupljenog otrova pčelinjim voskom i propolisom.
Abstract (english)
Bee venom (apitoxin) is a valuable honey bee (Apis melifera L.) product characterized by wide use and growing demand in pharmaceutical and cosmetic industry. It is predominantly consisted of peptides and enzymes (60-70%). The aim of this study was to perform a detailed chemical characterization of honey bee venom using infrared spectroscopy. Samples of bee venom were collected from 3 experimental honey bee colonies directly from the sting of worker bees of different age, and by bee electrostimulation method (pooled sample), and analysed by FTIR-ATR spectroscopy. The results have revealed that bee venom provides a characteristic FTIR-ATR spectral profile dominated by absorption bands of proteins (enzymes) and peptides. The results of the spectral analysis of bee venom of workers of different age (7, 14, 24 and 42 days old) showed that the qualitative and quantitative composition of the bee venom is stable throughout the life of the honey bee, while the analysis of a pooled venom sample collected from the experimental colonies indicated contamination of the collected venom with beeswax and propolis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:778601
Study programmeTitle: Plant Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2019-11-08 07:15:30