Biomass, as an important component of renewable energy sources, includes a wide range of raw materials for energy production. As a result, it is becoming an increasingly competitive renewable energy source that will contribute to the overall energy supply system, mainly due to its very low cost and the fact that it is renewable. In recent years, agricultural energy crops have shown great technical potential for such purposes. For the purpose of producing various forms of energy, several agricultural energy crops are used, and one of such crops is the perennial grass Miscanthus, which is the most researched in the EU. Due to its simple cultivation, high yields and low requirements and high energy properties, it is an ideal energy culture. In this research, 5 hybrids of M. sacchariflorus x M. sinensis were used and the energy properties of the investigated biomass was determined. The effect of moisture and hydrogen content on the heating value was also examined. Moisture in biomass generally decreases its heating value. Moreover, the high hydrogen content has a positive effect on the heating value and improves the quality of the energy source itself. Based on the obtained results it can be concluded that the higher heating value decreases with increasing moisture content. Furthermore, the lower heating value increases due to the increase in hydrogen content. The analyses confirmed the potential of Miscanthus grass as an energy-high-quality and environmentally friendly raw material for energy and fuel production.