| Abstract | Vinova loza kao najraširenija voćna kultura ima dugu povijest uzgajanja. U skorijoj povijesti otkriveni su i proučavani virusi koji mogu inficirati vinovu lozu. Ekonomski je značajno manji broj virusa, ali zaraženost njima ima drastične posljedice na vinovu lozu. U ovom radu istražena je pojavnost četiri ekonomski značajna virusa vinove loze na sorti Lasina. Uzorci su uzeti za vrijeme mirovanja vegetacije s bazalnog dijela trsa u obliku 2-3 dobro odrvenjele rozgve dužine 10 – 15 cm. Uzorci su prikupljeni na glavnom uzgojnom području sorte Lasina (lokacije Plastovo, Promina, Suhovare i Badanj). Testiranje je provedeno metodom ELISA i to na viruse infektivne degeneracije (virus mozaika gušarke – ArMV i virus lepezastog lista vinove loze - GFLV) te viruse iz skupine uvijenosti lista vinove loze (uvijenosti lista vinove loze pridruženi virusi 1 i 3, GLRaV-1 i GLRaV-3). Provedenim istraživanjem u kojem je testirano ukupno 120 trsova sa 4 lokacije nije potvrđena prisutnost ArMV, ali je utvrđena prisutnost ostalih virusa obuhvaćenih istraživanjem. Najzastupljeniji virus je bio GLRaV-3, koji je utvrđen u 84,17% uzoraka. U nešto manjoj zastupljenosti pronađen je GLRaV-1 (28,33%) te GFLV (5,83%). Osim pojedinačnih zaraza utvrđena je učestala pojava mješovitih zaraza s dva ili tri virusa obuhvaćenih istraživanjem, pri čemu su najučestalije bile kombinacije GLRaV-1+GLRaV-3 (22,5%) te GLRaV-3+GFLV (4,16%). Provedeno istraživanje ukazuje na narušeno zdravstveno stanje sorte Lasina u pogledu zaraženosti virusima i potrebu za intenziviranjem rada na njezinoj klonskoj i zdravstvenoj selekciji. |
| Abstract (english) | The grapevine, as the most widespread fruit crop, has a long history of cultivation. In recent history, viruses that can infect grapevines have been discovered and studied. Economically, the number of viruses is significantly lower, but infection with them has drastic consequences on the grapevine. In this study, the occurrence of four economically important grapevine viruses on the variety Lasina was investigated. The samples were taken during the dormancy from the basal part of each vine in the form of a 2-3 well-wooded cuttings 10-15 cm long. The samples were collected from main growing area of the variety Lasina (locations Plastovo, Promina, Suhovare and Badanj). Testing was carried out using the ELISA method for infectious degeneration viruses (arabis mosaic virus - ArMV and grapevine fanleaf virus - GFLV) and viruses from the leafroll complex (grapevine leafroll-associated virus viruses 1 and 3, GLRaV-1 and GLRaV -3). The conducted research in which a total of 120 vines from 4 location were examined, the presence of ArMV was not confirmed, but the presence of other viruses included in the research was determined. The most prevalent virus was GLRaV-3, which was found in 84,17% of samples. GLRaV-1 (28,33%) and GFLV (5,83%) were found in slightly lower abundance. In addition to individual infections, a frequent occurrence of mixed infections with two or three viruses included in the research was found, with the most frequent combinations being GLRaV-1+GLRaV-3 (22,5%) and GLRaV-3+GFLV (4,16%). The conducted research indicates the impaired health of the Lasina variety in terms of virus infection and the need to intensify work on its clonal and sanitary selection. |
