The grapevine, as the most widespread fruit crop, has a long history of cultivation. In recent history, viruses that can infect grapevines have been discovered and studied. Economically, the number of viruses is significantly lower, but infection with them has drastic consequences on the grapevine. In this study, the occurrence of four economically important grapevine viruses on the variety Lasina was investigated. The samples were taken during the dormancy from the basal part of each vine in the form of a 2-3 well-wooded cuttings 10-15 cm long. The samples were collected from main growing area of the variety Lasina (locations Plastovo, Promina, Suhovare and Badanj). Testing was carried out using the ELISA method for infectious degeneration viruses (arabis mosaic virus - ArMV and grapevine fanleaf virus - GFLV) and viruses from the leafroll complex (grapevine leafroll-associated virus viruses 1 and 3, GLRaV-1 and GLRaV -3). The conducted research in which a total of 120 vines from 4 location were examined, the presence of ArMV was not confirmed, but the presence of other viruses included in the research was determined. The most prevalent virus was GLRaV-3, which was found in 84,17% of samples. GLRaV-1 (28,33%) and GFLV (5,83%) were found in slightly lower abundance. In addition to individual infections, a frequent occurrence of mixed infections with two or three viruses included in the research was found, with the most frequent combinations being GLRaV-1+GLRaV-3 (22,5%) and GLRaV-3+GFLV (4,16%). The conducted research indicates the impaired health of the Lasina variety in terms of virus infection and the need to intensify work on its clonal and sanitary selection.