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master's thesis
In vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira
Zagreb: University of Zagreb, Faculty of Agriculture, 2023. urn:nbn:hr:204:123002
Rohrbacher, Valentina
University of Zagreb
Faculty of Agriculture
Department of Plant Breeding Genetics, Biometrics and Experimentation

Institutional repository: Repository Faculty of Agriculture University of Zagreb

Cite this document

Rohrbacher, V. (2023). In vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:123002

Rohrbacher, Valentina. "In vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira." Master's thesis, University of Zagreb, Faculty of Agriculture, 2023. https://urn.nsk.hr/urn:nbn:hr:204:123002

Rohrbacher, Valentina. "In vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira." Master's thesis, University of Zagreb, Faculty of Agriculture, 2023. https://urn.nsk.hr/urn:nbn:hr:204:123002

Rohrbacher, V. (2023). 'In vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 26 June 2024, https://urn.nsk.hr/urn:nbn:hr:204:123002

Rohrbacher V. In vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2023 [cited 2024 June 26] Available at: https://urn.nsk.hr/urn:nbn:hr:204:123002

V. Rohrbacher, "In vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:204:123002

Metadata
TitleIn vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira
Title (english)In vitro methods of elimination of potato virus M from traditional potato cultivar ‘Brinjak’
AuthorValentina Rohrbacher
MentorSnježana Kereša (mentor)
Committee memberSnježana Kereša (predsjednik povjerenstva)
Committee memberMilan Pospišil (član povjerenstva)
Committee memberDarko Vončina (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Department of Plant Breeding Genetics, Biometrics and Experimentation)
Zagreb
Defense date and country2023-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
'Brinjak' je tradicijski kultivar krumpira koji se generacijama uzgaja u Lici. Otporan je na plamenjaču i običnu krastavost krumpira, no zbog zaraze virusima u proizvodnji ne može postići svoj puni potencijal rodnosti. M-virus krumpira (eng. potato virus M, PVM) može smanjiti prinos gomolja od 11-45%. Primjenjujući tri različite in vitro metode pokušali smo eliminirati M-virus krumpira kojim je ovaj kultivar zaražen. Korištene su metode kulture izoliranih meristema bez prethodne terapije, kulture meristema nakon termoterapije na 37 C i kulture meristema nakon elektroterapije. Metodom ELISA ispitana je prisutnost M-virusa krumpira u listovima krumpira 'Brinjak' tretiranih trima navedenim metodama. Rezultati istraživanja su pokazali da je najveći postotak biljaka oslobođenih od M-virusa krumpira dobiven nakon tretmana termoterapijom u kombinaciji s kulturom meristema (94,1 %). Elektroterapijom u kombinaciji s kulturom meristema oslobođeno je 64,7 % uzoraka krumpira, a izolacijom meristema bez prethodnih tretmana 53,8 % prethodno zaraženih uzoraka kultivara 'Brinjak'.
Abstract (english)
‘Brinjak’ is a traditional potato cultivar that has been grown in Lika for generations. It is resistant to late blight and common potato scab. However, due to virus infection during production, it cannot achieve its full yield potential. PVM (Potato virus M) can reduce tuber yield by 11-45%. Applying three different in vitro methods, we tried to eliminate the PVM with which this cultivar was infected. The methods that were used were isolated meristems without previous therapy, meristems after thermotherapy at 37 C and meristems after electrotherapy. Then, the presence of PVM virus, in the leaves of cultivar ‘Brinjak’, was determined by using the ELISA method. The results showed that the highest percentage of virus-free plants of potato ‘Brinjak’ was obtained after treatment with thermotherapy in combination with meristem culture (94.1%). Electrotherapy in combination with meristem culture eliminated viruses from 64.7% of treated plants, while by the culture of isolated meristems without prior treatment PVM was eliminated from 53.8% of the plants.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:123002
Study programmeTitle: Plant Sciences
Study programme type: university
Study level: graduate
Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-09-25 08:34:46