prikaz prve stranice dokumenta Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica
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master's thesis
Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica
Zagreb: University of Zagreb, Faculty of Agriculture, 2023. urn:nbn:hr:204:495688
Kolar, Sara
University of Zagreb
Faculty of Agriculture
Animal Nutrition

Institutional repository: Repository Faculty of Agriculture University of Zagreb

Cite this document

Kolar, S. (2023). Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:495688

Kolar, Sara. "Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica." Master's thesis, University of Zagreb, Faculty of Agriculture, 2023. https://urn.nsk.hr/urn:nbn:hr:204:495688

Kolar, Sara. "Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica." Master's thesis, University of Zagreb, Faculty of Agriculture, 2023. https://urn.nsk.hr/urn:nbn:hr:204:495688

Kolar, S. (2023). 'Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:204:495688

Kolar S. Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2023 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:204:495688

S. Kolar, "Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:204:495688

Metadata
TitleUsporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica
Title (english)Comparison of standardized infogest in vitro digestibility method and in vivo digestion of carotenoids in laying hens
AuthorSara Kolar
MentorKristina Kljak (mentor)
Committee memberKristina Kljak (predsjednik povjerenstva)
Committee memberGoran Kiš (član povjerenstva)
Committee memberKrešimir Salajpal (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Agriculture
(Animal Nutrition)
Zagreb
Defense date and country2023-09-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract
Jedina standardizirana in vitro metoda probavljivosti je INFOGEST metoda, no do sada nije ispitano koliko je precizna u određivanju probavljivosti karotenoida. Cilj ovog rada je bio usporediti rezultate standardizirane in vitro i in vivo metode probavljivosti karotenoida u kokoši nesilica hranjenih dvjema potpunim krmnim smjesama koje se razlikuju u hibridu kukuruza kao jedinim izvorom karotenoida. U in vivo pokusu uzorkovani su sadržaji duodenuma, jejunuma, ileuma i debelog crijeva nesilica, dok je INFOGEST metoda provedena s inkubacijo m od 2,5; 3; 3,5; 4; 4,5 i 5 sati. Usporedivi podatci između in vitro i in vivo metoda utvrđeni su za lutein kojem su vrijednosti nakon 3; 3,5 i 4,5 sati inkubacije slične vrijednostima u jejunumu (P=0,60), ileumu (P=0,87) i debelom crijevu (P=0,42). Kod ostalih karotenoida, usporedivi podatci su bili za probavljivost zeaksantina i u kupnih karotenoida i to između inkubacije nakon 4 sata i jejunuma (P=0,97 i 0,59). Dobiveni podatci pokazuju da je potrebno dodatno prilagoditi INFOGEST metodu da bude prikladna za probavljivost karotenoida.
Abstract (english)
The only standardised in vitro digestiblity method is the INFOGEST method, but so far it has not been tested how accurately it determines the digestibility of carotenoids. The aim of this research was to compare the results of the INFOGEST method and in vi vo data on the digestibility of carotenoids in laying hens fed two complete feed mixtures differing in the maize hybrid as the only source of carotenoids. In the in vivo experiment, the contents of the duodenum, jejunum, ileum and colon of the hens were sa mpled, while the INFOGEST method was performed with incubation times of 2.5, 3, 3.5, 4, 4.5 and 5 hours. Comparable da t a between the in vitro and in vivo methods were obtained for lutein, whose values after 3, 3.5 and 4.5 hours of incubation were comparable with digestibility in the jejunum (P=0.60), ileum (P=0.87) and colon (P=0.42). For other carotenoids, comparable da t a were obtained for the digestib i lity of zeaxanthin and total carotenoids between incubation after 4 hours and digestibility in the jejunu m (P=0.97 and 0.59). The da t a obtained show that it is necessary to make additional adjustments to the INFOGEST method to ensure suitability for digestibility of carotenoids.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:204:495688
Study programmeTitle: Animal Nutrition and Feed Science
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka hranidbe životinja i hrane (magistar/ magistra inženjer/inženjerka hranidbe životinja i hrane)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to authenticated users
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Created on2023-09-27 08:48:12