prikaz prve stranice dokumenta Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica
  Download
PDF 13 MB
Scientific paper - Original scientific paper
Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica
Frontiers in Cellular Neuroscience, 15 (2021); 661492. https://doi.org/10.3389/fncel.2021.661492
Petrović, Antonela; Ban, Jelena; Tomljanović, Ivana; Pongrac, Marta; Ivaničić, Matea; Mikašinović, Sanja; Mladinic, Miranda

Institutional repository: Repository of the University of Rijeka, Faculty of Biotechnology and Drug Development

Cite this document

Petrović, A., Ban, J., Tomljanović, I., Pongrac, M., Ivaničić, M., Mikašinović, S. & Mladinic, M. (2021). Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica. Frontiers in Cellular Neuroscience, 15.. doi: 10.3389/fncel.2021.661492

Petrović, Antonela, et al. "Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica." Frontiers in Cellular Neuroscience, vol. 15, 2021. https://doi.org/10.3389/fncel.2021.661492

Petrović, Antonela, Jelena Ban, Ivana Tomljanović, Marta Pongrac, Matea Ivaničić, Sanja Mikašinović and Miranda Mladinic. "Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica." Frontiers in Cellular Neuroscience 15 (2021). https://doi.org/10.3389/fncel.2021.661492

Petrović, A., et al. (2021) 'Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica', Frontiers in Cellular Neuroscience, 15. doi: 10.3389/fncel.2021.661492

Petrović A, Ban J, Tomljanović I, Pongrac M, Ivaničić M, Mikašinović S, and sur.. Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica. Frontiers in Cellular Neuroscience [Internet]. 2021 March 18 [cited 2024 April 25];15. doi: 10.3389/fncel.2021.661492

A. Petrović, et al., "Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica", Frontiers in Cellular Neuroscience, vol. 15, March 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:193:462045. [Accessed: 25 April 2024]

Metadata
Title (english)Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica
AuthorAntonela Petrović
AuthorJelena Ban
AuthorIvana Tomljanović
AuthorMarta Pongrac
AuthorMatea Ivaničić
AuthorSanja Mikašinović
AuthorMiranda Mladinic
Author's institutionUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Abstract (english)
Primary dissociated neuronal cultures have become a standard model for studying central nervous system (CNS) development. Such cultures are predominantly prepared from the hippocampus or cortex of rodents (mice and rats), while other mammals are less used. Here, we describe the establishment and extensive characterization of the primary dissociated neuronal cultures derived from the cortex of the gray South American shorttailed opossums, Monodelphis domestica. Opossums are unique in their ability to fully regenerate their CNS after an injury during their early postnatal development. Thus, we used cortex of postnatal day (P) 3–5 opossum to establish long-surviving and nearly pure neuronal cultures, as well as mixed cultures composed of radial glia cells (RGCs) in which their neurogenic and gliogenic potential was confirmed. Both types of cultures can survive for more than 1 month in vitro. We also prepared neuronal cultures from the P16–18 opossum cortex, which were composed of astrocytes and microglia, in addition to neurons. The long-surviving opossum primary dissociated neuronal cultures represent a novel mammalian in vitro platform particularly useful to study CNS development and regeneration.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleFrontiers in Cellular Neuroscience
Numberingvol. 15, 661492
p-ISSN1662-5102
DOIhttps://doi.org/10.3389/fncel.2021.661492
URN:NBNurn:nbn:hr:193:462045
Publication2021-03-18
ProjectNumber: HRZZ-IP-2016-06-7060
Title (croatian): Istraživanje granice između neurodegeneracije i neuroregeneracije: identifikacija ključnih molekula pomoću proteomike i funkcionalnih testova na leđnoj moždini sisavaca
Acronym: DefineREgenAgeMode
Leader: Miranda Mladinić Pejatović
Jurisdiction: Croatia
Document URLhttps://www.frontiersin.org/articles/10.3389/fncel.2021.661492/full
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-02-02 20:29:02