prikaz prve stranice dokumenta Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica
  Preuzmi
PDF 13 MB
Znanstveni rad - Izvorni znanstveni rad
Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica
Frontiers in Cellular Neuroscience, 15 (2021); 661492. https://doi.org/10.3389/fncel.2021.661492
Petrović, Antonela; Ban, Jelena; Tomljanović, Ivana; Pongrac, Marta; Ivaničić, Matea; Mikašinović, Sanja; Mladinic, Miranda

Institucijski repozitorij: Repozitorij Fakulteta biotehnologije i razvoja lijekova

Citirajte ovaj rad

Petrović, A., Ban, J., Tomljanović, I., Pongrac, M., Ivaničić, M., Mikašinović, S. i Mladinic, M. (2021). Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica. Frontiers in Cellular Neuroscience, 15.. doi: 10.3389/fncel.2021.661492

Petrović, Antonela, et al. "Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica." Frontiers in Cellular Neuroscience, vol. 15, 2021. https://doi.org/10.3389/fncel.2021.661492

Petrović, Antonela, Jelena Ban, Ivana Tomljanović, Marta Pongrac, Matea Ivaničić, Sanja Mikašinović i Miranda Mladinic. "Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica." Frontiers in Cellular Neuroscience 15 (2021). https://doi.org/10.3389/fncel.2021.661492

Petrović, A., et al. (2021) 'Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica', Frontiers in Cellular Neuroscience, 15. doi: 10.3389/fncel.2021.661492

Petrović A, Ban J, Tomljanović I, Pongrac M, Ivaničić M, Mikašinović S, i sur.. Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica. Frontiers in Cellular Neuroscience [Internet]. 18.03.2021. [pristupljeno 25.04.2024.];15. doi: 10.3389/fncel.2021.661492

A. Petrović, et al., "Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica", Frontiers in Cellular Neuroscience, vol. 15, Ožujak 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:193:462045. [Citirano: 25.04.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:193:462045
Podaci o radu
Naslov (engleski)Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica
AutorAntonela Petrović
AutorJelena Ban
AutorIvana Tomljanović
AutorMarta Pongrac
AutorMatea Ivaničić
AutorSanja Mikašinović
AutorMiranda Mladinic
Autorova ustanovaSveučilište u Rijeci
(Fakultet biotehnologije i razvoja lijekova)
Znanstveno / umjetničko
područje, polje i grana		INTERDISCIPLINARNA PODRUČJA ZNANOSTI
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Sažetak (engleski)
Primary dissociated neuronal cultures have become a standard model for studying central nervous system (CNS) development. Such cultures are predominantly prepared from the hippocampus or cortex of rodents (mice and rats), while other mammals are less used. Here, we describe the establishment and extensive characterization of the primary dissociated neuronal cultures derived from the cortex of the gray South American shorttailed opossums, Monodelphis domestica. Opossums are unique in their ability to fully regenerate their CNS after an injury during their early postnatal development. Thus, we used cortex of postnatal day (P) 3–5 opossum to establish long-surviving and nearly pure neuronal cultures, as well as mixed cultures composed of radial glia cells (RGCs) in which their neurogenic and gliogenic potential was confirmed. Both types of cultures can survive for more than 1 month in vitro. We also prepared neuronal cultures from the P16–18 opossum cortex, which were composed of astrocytes and microglia, in addition to neurons. The long-surviving opossum primary dissociated neuronal cultures represent a novel mammalian in vitro platform particularly useful to study CNS development and regeneration.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaFrontiers in Cellular Neuroscience
Brojčani podacivol. 15, 661492
p-ISSN1662-5102
DOIhttps://doi.org/10.3389/fncel.2021.661492
URN:NBNurn:nbn:hr:193:462045
Datum objave publikacije2021-03-18
ProjektŠifra: HRZZ-IP-2016-06-7060
Naziv (hrvatski): Istraživanje granice između neurodegeneracije i neuroregeneracije: identifikacija ključnih molekula pomoću proteomike i funkcionalnih testova na leđnoj moždini sisavaca
Kratica: DefineREgenAgeMode
Voditelj: Miranda Mladinić Pejatović
Pravna nadležnost: Hrvatska
Financijer: HRZZ
URL dokumentahttps://www.frontiersin.org/articles/10.3389/fncel.2021.661492/full
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2022-02-02 20:29:02