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master's thesis
Ekstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. urn:nbn:hr:149:238036
Opačak, Andrea
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Opačak, A. (2023). Ekstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala (Master's thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:238036

Opačak, Andrea. "Ekstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:149:238036

Opačak, Andrea. "Ekstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala." Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:149:238036

Opačak, A. (2023). 'Ekstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala', Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:149:238036

Opačak A. Ekstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala [Master's thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2023 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:149:238036

A. Opačak, "Ekstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala", Master's thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:149:238036

Metadata
TitleEkstrakcija piperina iz različitih vrsta papra: odabir ekološki prihvatljivog otapala
Title (english)Extraction of piperine from different types of peppers: green solvent screening
AuthorAndrea Opačak
MentorAleksandra Sander (mentor)
Committee memberAleksandra Sander (predsjednik povjerenstva)
Committee memberDajana Kučić Grgić (član povjerenstva)
Committee memberJasna Prlić Kardum (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2023-01-27, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Kako bi se postigli ciljevi održivog razvoja, razvoj novih zelenih otapala trenutno je u žarištu istraživanja. Zbog lakog procesa pripreme, niske cijene, potencijalne biorazgradivosti i zanemarive toksičnosti niskotemperaturna eutektička otapala (engl. Deep Eutentic Solvents (DES)) prepoznata su kao dobra zamjena organskim otapalima. Zahvaljujući sve većem interesu i razvoju niskotemperaturnih eutektičkih otapala sve se više primjenjuju kao mediji za ekstrakciju, kromatografiju te u biomedicinske svrhe u proteklih nekoliko godina. Cilj ovog rada bio je utvrditi koje niskotemperaturno eutektičko otapalo je najpogodnije za ekstrakciju piperina iz bijelog i zelenog papra. Zrna papra usitnjena su i prosijana te su dobivene tri veličinske frakcije. Piperin je nepolarna molekula te su zbog toga za ekstrakciju su pripravljena četiri hidrofobna otapala, od kojih su dva na bazi timola te dva na bazi mentola. Modelom COSMO-RS predviđena je topljivost piperina u pripremljenim otapalima te je provedena ekstrakcija sa svim veličinskim frakcijama papra. Koncentracija piperina u dobivenim uzorcima utvrđena je UV/Vis spektroskopijom, bez prethodne obrade ekstrakata. Također, disk-difuzijskim testom ispitano je antimikrobno djelovanje ekstrakata, a test toksičnosti čistih otapala proveden je pomoću bioluminiscentne bakterije Vibrio fischeri.
Abstract (english)
In order to achieve the goals of sustainable development, the development of new green solvents is currently in the focus of research. Due to the easy preparation process, low price, potential biodegradability and negligible toxicity, Deep Eutentic Solvents (DES) are recognized as a good substitute for organic solvents. Thanks to the increasing interest and development of deep eutentic solvents, it is increasingly used as a medium for extraction, chromatography and for biomedical purposes in the past few years. The aim of this work was to determine which deep eutentic solvent is most suitable for the extraction of piperine from white and green pepper. The peppercorns were crushed and sieved, and three size fractions were obtained. Piperine is a non-polar molecule, which is why four hydrophobic solvents are prepared for extraction, two of them are thymol-based and two are menthol-based. The solubility of piperine in the prepared solvents was predicted by the COSMO-RS model, and extraction was performed with all size fractions of pepper. The concentration of piperine in the obtained samples was determined by UV/Vis spectroscopy, without prior processing of the extracts. Also, the antimicrobial activity of the extracts was tested with the disk diffusion test, and the toxicity test of pure solvents was performed using the bioluminescent bacterium Vibrio fischeri.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:238036
Study programmeTitle: Environmental Engineering - Graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka ekoinženjerstva (magistar/magistra inženjer/inženjerka ekoinženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-02-15 11:43:16