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undergraduate thesis
Aktivnost ketoreduktaza pri oksidaciji diola
Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology, 2017. urn:nbn:hr:149:514634
Matković, Valerija
University of Zagreb
Faculty of Chemical Engineering and Technology

Institutional repository: Repository of Faculty of Chemical Engineering and Technology University of Zagreb

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Matković, V. (2017). Aktivnost ketoreduktaza pri oksidaciji diola (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:149:514634

Matković, Valerija. "Aktivnost ketoreduktaza pri oksidaciji diola." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2017. https://urn.nsk.hr/urn:nbn:hr:149:514634

Matković, Valerija. "Aktivnost ketoreduktaza pri oksidaciji diola." Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, 2017. https://urn.nsk.hr/urn:nbn:hr:149:514634

Matković, V. (2017). 'Aktivnost ketoreduktaza pri oksidaciji diola', Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, accessed 18 May 2024, https://urn.nsk.hr/urn:nbn:hr:149:514634

Matković V. Aktivnost ketoreduktaza pri oksidaciji diola [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Chemical Engineering and Technology; 2017 [cited 2024 May 18] Available at: https://urn.nsk.hr/urn:nbn:hr:149:514634

V. Matković, "Aktivnost ketoreduktaza pri oksidaciji diola", Undergraduate thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:149:514634

Metadata
TitleAktivnost ketoreduktaza pri oksidaciji diola
Title (english)Activity of ketoreductases during diol oxidation
AuthorValerija Matković
MentorZvjezdana Findrik Blažević (mentor)
Committee memberZvjezdana Findrik Blažević (predsjednik povjerenstva)
Committee memberMartina Sudar (član povjerenstva)
Committee memberMarija Vuković Domanovac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Chemical Engineering and Technology
Zagreb
Defense date and country2017-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Reaction Engineering
Abstract
U radu je opisana kaskadna reakcija koja objedinjuje reakciju oksidacije Cbz-N-3-amino-1,2-propandiola (diol) te reakciju aldolne adicije dihidroksiacetona (DHA) na aldehid sintetiziran u koraku oksidacije. Za reakciju oksidacije testirano je 18 ketoreduktaza, te je njihova aktivnost uspoređena s aktivnošću enzima alkohol dehidrogenaze iz jetre konja (HLADH) koji se ranije pokazao kao katalizator aktivan u proučavanoj oksidoredukciji. Ketoreduktaze i dehidrogenaze zahtijevaju prisutnost koenzima NAD+ ili NADP+. Među testiranim enzimima pronađena su 2 aktivna uz NAD+, te tri aktivna uz koenzim NADP+ u proučavanoj oksidoredukciji. Odabrani enzimi su korišteni za provedbu kaskadne reakcije u kojoj je nakon oksidoredukcije slijedila aldolna adicija dihidroksiacetona na aldehid katalizirana D-fruktoza-6-fosfat aldolazom (FSA). Za učinkovitu oksidaciju diola korištena je NADH oksidaza (NOX) za regeneraciju koenzima. Kaskadna reakcija je praćena preko koncentracije nastalog aldolnog produkta nakon 24 i 48 sati, koja je ujedno bila korištena i kao mjerilo uspješnosti provedene reakcije. Usprkos tomu što je u provedenim reakcijama nastao željeni produkt, zaključeno je da je među ispitanima HLADH najbolji katalizator za navedenu reakciju.
Abstract (english)
This paper describes a cascade reaction which combines the oxidation reaction of Cbz-N-3-amino-1,2-propanediol (diol) and the reaction of the aldol addition of dihydroxyacetone (DHA) to the aldehyde synthesized in the oxidation step. 18 ketoreductases were tested for oxidation reaction, and their activity was compared with the activity of alcohol dehydrogenase from horse liver (HLADH), which previously proved to be the catalyst active in the study of oxidation. Ketoreductases and dehydrogenases require the presence of NAD+ or NADP+ coenzyme. Among the tested enzymes, 2 were active in the studied reaction with NAD+, and three with coenzyme NADP+. The selected enzymes were used to carry out a cascade reaction in which following oxidation, the aldol addition of dihydroxyacetone to aldehyde catalyzed by D-fructose-6-phosphate aldolase (FSA) was carried out. NADH oxidase (NOX) for coenzyme regeneration was used for the effective oxidation of diol. The concentration of the formed aldol product after 24 and 48 hours in the cascade reaction was followed and was used as a measure of the reaction effectiveness. In spite of the fact that the desired product was formed in all reactions, HLADH was still found to be the best catalyst for this reaction.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:149:514634
ProjectNumber: 635595
Title: Sustainable industrial processes based on a C-C bond-forming enzyme platform
Acronym: CARBAZYMES
Leader: Zvjezdana Findrik Blažević
Jurisdiction: eu
Funder: EC
Funding stream: H2020
Study programmeTitle: Chemical Engineering - Undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2021-03-23 09:53:17