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master's thesis
Inhibicijski učinak odabranih biljnih ekstrakata na aktivnost enzima α-glukozidaza
diplomski rad
Split: University of Split, Faculty of Chemistry and Technology, 2019. urn:nbn:hr:167:750004
Miletić, Vicenzia
University of Split
Faculty of Chemistry and Technology
Division of Chemistry
Department of Biochemistry

Institutional repository: Repository of the Faculty of chemistry and technology

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Miletić, V. (2019). Inhibicijski učinak odabranih biljnih ekstrakata na aktivnost enzima α-glukozidaza : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:750004

Miletić, Vicenzia. "Inhibicijski učinak odabranih biljnih ekstrakata na aktivnost enzima α-glukozidaza : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:167:750004

Miletić, Vicenzia. "Inhibicijski učinak odabranih biljnih ekstrakata na aktivnost enzima α-glukozidaza : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:167:750004

Miletić, V. (2019). 'Inhibicijski učinak odabranih biljnih ekstrakata na aktivnost enzima α-glukozidaza : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 20 May 2024, https://urn.nsk.hr/urn:nbn:hr:167:750004

Miletić V. Inhibicijski učinak odabranih biljnih ekstrakata na aktivnost enzima α-glukozidaza : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2019 [cited 2024 May 20] Available at: https://urn.nsk.hr/urn:nbn:hr:167:750004

V. Miletić, "Inhibicijski učinak odabranih biljnih ekstrakata na aktivnost enzima α-glukozidaza : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:167:750004

Metadata
TitleInhibicijski učinak odabranih biljnih ekstrakata na aktivnost enzima α-glukozidaza : diplomski rad
Title (english)Inhibitory effect selected plant extracts on α-glucosidase activity : diploma thesis
AuthorVicenzia Miletić
MentorOlivera Politeo (mentor)
Committee memberOlivera Politeo (predsjednik povjerenstva)
Committee memberJosipa Giljanović (član povjerenstva)
Committee memberMila Radan (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
(Department of Biochemistry)
Split
Defense date and country2019-10-15, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Šećerna bolest je rastući javnozdravstveni problem. Mnoga provedena istraživanja potvrdila su dobrobiti ljekovitih biljaka s hipoglikemijskim učinkom u liječenju dijabetesa. Učinak ovih biljaka može odgoditi razvoj komplikacija šećerne bolesti te ispraviti metaboličke nepravilnosti. Cilj ovog rada bio je testirati sposobnost eteričnih ulja i hidrolata odabranih biljnih vrsta da inhibiraju enzim α-glukozidazu, metodom po Sigma-Aldrichu. Za uzorke hidrolata je određen i sadržaj ukupnih fenola metodom po Folin- Ciocalteu. Rezultati su pokazali da je najučinkovitiji inhibitor među testiranim uzorcima hidrolat biljke Juniperus communis spp. nana (IC 50 =47,89 mg/L). Ostali uzorci biljaka za sve testirane koncentracije slabo inhibiraju enzim α-glukozidazu. Među eteričnim uljima najbolji inhibitor enzima α-glukozidaze je ulje biljke Hymenocarpos circinnatus koje u koncentraciji 1 g/L inhibira enzim s 52,16±3,82 %. Nešto lošiji učinak kod iste koncentracije imaju Centaurea scabiosa s 38,72±11,05 % i Mantisalca salmantica 32,2±12,72 %, dok eterično ulje biljke Campanula rapunculus nije pokazalo inhibicijski učinak na ispitivani enzim. Najučinkovitiji inhibitor, hidrolat biljke Juniperus communis spp. nana sadrži i najviše fenolnih spojeva (196 mg GAE/g hidrolata), dok za ostale testirane hidrolate nema značajne povezanosti između sadržaja ukupnih fenola i inhibicije enzima.
Abstract (english)
Diabetes is a growing public health problem. Many studies have confirmed the benefits of medicinal plants with a hypoglycemic effect in the treatment of diabetes. The effect of these herbs can delay the development of diabetes complications and correct metabolic abnormalities. The aim of this study was to test the ability of essential oils and hydrolats of selected plant species to inhibit the α-glucosidase enzyme, using the Sigma-Aldrich method. The content of total phenolics was determined by the Folin-Ciocalteu method for the hydrolate samples. The results showed that the most effective inhibitor among the tested hydrolats was that of the Juniperus communis spp. nana plant (IC 50 =47.89 mg/L). Other samples at all tested concentrations weakly inhibit the enzyme. Among essential oils, the best inhibitor of α-glucosidase enzyme is the oil of the plant Hymenocarpos circinnatus, which at a concentration of 1 g/L inhibits the enzyme by 52.16 ± 3.82 %. Centaurea scabiosa with 38.72±11.05 % and Mantisalca salmantica with 32.2±12.72 % had a slightly lower inhibitory effect at the same concentration, while the essential oil of Campanula rapunculus showed no inhibitory effect on this enzyme. The highest total phenolics content was found in most effective inhibitor; hydrolate of the plant Juniperus communis spp. nana (196 mg GAE/g hydrolate), while for the other tested hydrolats there is no significant correlation between total phenolics content and enzyme inhibition.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:750004
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2021-10-15
Terms of useLicence In copyright icon
Created on2020-07-14 08:37:32