prikaz prve stranice dokumenta The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages
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Scientific paper - Original scientific paper
The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages
PLoS Pathogens, 12 (2016), 12; e1006057. https://doi.org/10.1371/journal.
Thiel, Nadine; Keyser, Kirsten A.; Lemmermann, Niels A.W.; Oduro, Jennifer D.; Wagner, Karen; Elsner, Carina; Halenius, Anne; Lenac Roviš, Tihana; Brinkmann, Melanie M.; Jonjić, Stipan; Čičin-Šain, Luka; Messerle, Martin

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Thiel, N., Keyser, K. A., Lemmermann, N. A., Oduro, J. D., Wagner, K., Elsner, C. ... Messerle, M. (2016). The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages. PLoS Pathogens, 12. (12). doi: 10.1371/journal.

Thiel, Nadine, et al. "The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages." PLoS Pathogens, vol. 12, no. 12, 2016. https://doi.org/10.1371/journal.

Thiel, Nadine, Kirsten A. Keyser, Niels A.W. Lemmermann, Jennifer D. Oduro, Karen Wagner, Carina Elsner, Anne Halenius, et al. "The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages." PLoS Pathogens 12, no. 12 (2016). https://doi.org/10.1371/journal.

Thiel, N., et al. (2016) 'The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages', PLoS Pathogens, 12(12). doi: 10.1371/journal.

Thiel N, Keyser KA, Lemmermann NA, Oduro JD, Wagner K, Elsner C, and sur.. The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages. PLoS Pathogens [Internet]. 2016 December 07 [cited 2024 May 14];12(12). doi: 10.1371/journal.

N. Thiel, et al., "The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages", PLoS Pathogens, vol. 12, no. 12, December 2016. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:198883. [Accessed: 14 May 2024]

Metadata
Title (english)The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages
AuthorNadine Thiel
AuthorKirsten A. Keyser
AuthorNiels A.W. Lemmermann
AuthorJennifer D. Oduro
AuthorKaren Wagner
AuthorCarina Elsner
AuthorAnne Halenius
AuthorTihana Lenac Roviš
AuthorMelanie M. Brinkmann
AuthorStipan Jonjić
AuthorLuka Čičin-Šain
AuthorMartin Messerle
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
The receptor-like protein tyrosine phosphatase CD45 is expressed on the surface of cells of hematopoietic origin and has a pivotal role for the function of these cells in the immune response. Here we report that following infection of macrophages with mouse cytomegalovirus (MCMV) the cell surface expression of CD45 is drastically diminished. Screening of a set of MCMV deletion mutants allowed us to identify the viral gene m42 of being responsible for CD45 down-modulation. Moreover, expression of m42 independent of viral infection upon retroviral transduction of the RAW264.7 macrophage cell line led to comparable regulation of CD45 expression. In immunocompetent mice infected with an m42 deletion mutant lower viral titers were observed in all tissues examined when compared to wildtype MCMV, indicating an important role of m42 for viral replication in vivo. The m42 gene product was identified as an 18 kDa protein expressed with early kinetics and is predicted to be a tailanchored membrane protein. Tracking of surface-resident CD45 molecules revealed that m42 induces internalization and degradation of CD45. The observation that the amounts of the E3 ubiquitin ligases Itch and Nedd4 were diminished in cells expressing m42 and that disruption of a PY motif in the N-terminal part of m42 resulted in loss of function, suggest that m42 acts as an activator or adaptor for these Nedd4-like ubiquitin ligases, which mark CD45 for lysosomal degradation. In conclusion, the down-modulation of CD45 expression in MCMV-infected myeloid cells represents a novel pathway of virus-host interaction.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titlePLoS Pathogens
Numberingvol. 12, no. 12, e1006057
p-ISSN1553-7366
e-ISSN1553-7374
DOIhttps://doi.org/10.1371/journal.
URN:NBNurn:nbn:hr:184:198883
Publication2016-12-07
ProjectNumber: 322693
Title (english): Strengthening adaptive immunity via innate immunity: enhancing the CD8 T cell response by using the NKG2D ligand expressed in a herpesvirus vector
Acronym: STADVINN
Jurisdiction: eu
Funding stream: FP7
ProjectNumber: 260934
Title (english): Molecular mechanisms of persistent antigenic stimulation in cytomegalovirus infection
Acronym: CMVAGSTIMULUS
Jurisdiction: eu
Funding stream: FP7
Document URLhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142792/pdf/ppat.1006057.pdf
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-06-20 13:28:18