prikaz prve stranice dokumenta The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages
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The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages
PLoS Pathogens, 12 (2016), 12; e1006057. https://doi.org/10.1371/journal.
Thiel, Nadine; Keyser, Kirsten A.; Lemmermann, Niels A.W.; Oduro, Jennifer D.; Wagner, Karen; Elsner, Carina; Halenius, Anne; Lenac Roviš, Tihana; Brinkmann, Melanie M.; Jonjić, Stipan; Čičin-Šain, Luka; Messerle, Martin

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

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Thiel, N., Keyser, K. A., Lemmermann, N. A., Oduro, J. D., Wagner, K., Elsner, C. ... Messerle, M. (2016). The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages. PLoS Pathogens, 12. (12). doi: 10.1371/journal.

Thiel, Nadine, et al. "The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages." PLoS Pathogens, vol. 12, br. 12, 2016. https://doi.org/10.1371/journal.

Thiel, Nadine, Kirsten A. Keyser, Niels A.W. Lemmermann, Jennifer D. Oduro, Karen Wagner, Carina Elsner, Anne Halenius, et al. "The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages." PLoS Pathogens 12, br. 12 (2016). https://doi.org/10.1371/journal.

Thiel, N., et al. (2016) 'The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages', PLoS Pathogens, 12(12). doi: 10.1371/journal.

Thiel N, Keyser KA, Lemmermann NA, Oduro JD, Wagner K, Elsner C, i sur.. The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages. PLoS Pathogens [Internet]. 07.12.2016. [pristupljeno 24.12.2024.];12(12). doi: 10.1371/journal.

N. Thiel, et al., "The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages", PLoS Pathogens, vol. 12, br. 12, Prosinac 2016. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:198883. [Citirano: 24.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:198883
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Naslov (engleski)The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages
AutorNadine Thiel
AutorKirsten A. Keyser
AutorNiels A.W. Lemmermann
AutorJennifer D. Oduro
AutorKaren Wagner
AutorCarina Elsner
AutorAnne Halenius
AutorTihana Lenac Roviš
AutorMelanie M. Brinkmann
AutorStipan Jonjić
AutorLuka Čičin-Šain
AutorMartin Messerle
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
The receptor-like protein tyrosine phosphatase CD45 is expressed on the surface of cells of hematopoietic origin and has a pivotal role for the function of these cells in the immune response. Here we report that following infection of macrophages with mouse cytomegalovirus (MCMV) the cell surface expression of CD45 is drastically diminished. Screening of a set of MCMV deletion mutants allowed us to identify the viral gene m42 of being responsible for CD45 down-modulation. Moreover, expression of m42 independent of viral infection upon retroviral transduction of the RAW264.7 macrophage cell line led to comparable regulation of CD45 expression. In immunocompetent mice infected with an m42 deletion mutant lower viral titers were observed in all tissues examined when compared to wildtype MCMV, indicating an important role of m42 for viral replication in vivo. The m42 gene product was identified as an 18 kDa protein expressed with early kinetics and is predicted to be a tailanchored membrane protein. Tracking of surface-resident CD45 molecules revealed that m42 induces internalization and degradation of CD45. The observation that the amounts of the E3 ubiquitin ligases Itch and Nedd4 were diminished in cells expressing m42 and that disruption of a PY motif in the N-terminal part of m42 resulted in loss of function, suggest that m42 acts as an activator or adaptor for these Nedd4-like ubiquitin ligases, which mark CD45 for lysosomal degradation. In conclusion, the down-modulation of CD45 expression in MCMV-infected myeloid cells represents a novel pathway of virus-host interaction.
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaPLoS Pathogens
Brojčani podacivol. 12, br. 12, e1006057
p-ISSN1553-7366
e-ISSN1553-7374
DOIhttps://doi.org/10.1371/journal.
URN:NBNurn:nbn:hr:184:198883
Datum objave publikacije2016-12-07
ProjektŠifra: 322693
Naziv (engleski): Strengthening adaptive immunity via innate immunity: enhancing the CD8 T cell response by using the NKG2D ligand expressed in a herpesvirus vector
Kratica: STADVINN
Pravna nadležnost: eu
Financijer: EC
Financijer: EC
Financijer: EC
Financijer: EC
Linija financiranja: FP7
ProjektŠifra: 260934
Naziv (engleski): Molecular mechanisms of persistent antigenic stimulation in cytomegalovirus infection
Kratica: CMVAGSTIMULUS
Pravna nadležnost: eu
Financijer: EC
Financijer: EC
Financijer: EC
Linija financiranja: FP7
URL dokumentahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5142792/pdf/ppat.1006057.pdf
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2017-06-20 13:28:18