prikaz prve stranice dokumenta Varicella zoster virus infection of highly pure terminally differentiated human neurons
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Scientific paper - Original scientific paper
Varicella zoster virus infection of highly pure terminally differentiated human neurons
Journal of NeuroVirology, 19 (2013), 1; 75-81. https://doi.org/10.1007/s13365-012-0142-x
Yu, Xiaoli; Seitz, Scott; Pointon, Tiffany; Bowlin, Jacqueline L.; Cohrs, Randall J.; Jonjić, Stipan; Haas, Jürgen; Wellish, Mary; Gilden, Don

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Yu, X., Seitz, S., Pointon, T., Bowlin, J. L., Cohrs, R. J., Jonjić, S. ... Gilden, D. (2013). Varicella zoster virus infection of highly pure terminally differentiated human neurons. Journal of NeuroVirology, 19. (1), 75-81. doi: 10.1007/s13365-012-0142-x

Yu, Xiaoli, et al. "Varicella zoster virus infection of highly pure terminally differentiated human neurons." Journal of NeuroVirology, vol. 19, no. 1, 2013, pp. 75-81. https://doi.org/10.1007/s13365-012-0142-x

Yu, Xiaoli, Scott Seitz, Tiffany Pointon, Jacqueline L. Bowlin, Randall J. Cohrs, Stipan Jonjić, Jürgen Haas, Mary Wellish and Don Gilden. "Varicella zoster virus infection of highly pure terminally differentiated human neurons." Journal of NeuroVirology 19, no. 1 (2013): 75-81. https://doi.org/10.1007/s13365-012-0142-x

Yu, X., et al. (2013) 'Varicella zoster virus infection of highly pure terminally differentiated human neurons', Journal of NeuroVirology, 19(1), pp. 75-81. doi: 10.1007/s13365-012-0142-x

Yu X, Seitz S, Pointon T, Bowlin JL, Cohrs RJ, Jonjić S, and sur.. Varicella zoster virus infection of highly pure terminally differentiated human neurons. Journal of NeuroVirology [Internet]. 2013 December 12 [cited 2024 November 26];19(1):75-81. doi: 10.1007/s13365-012-0142-x

X. Yu, et al., "Varicella zoster virus infection of highly pure terminally differentiated human neurons", Journal of NeuroVirology, vol. 19, no. 1, pp. 75-81, December 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:750399. [Accessed: 26 November 2024]

Metadata
Title (english)Varicella zoster virus infection of highly pure terminally differentiated human neurons
AuthorXiaoli Yu
AuthorScott Seitz
AuthorTiffany Pointon
AuthorJacqueline L. Bowlin
AuthorRandall J. Cohrs
AuthorStipan Jonjić
AuthorJürgen Haas
AuthorMary Wellish
AuthorDon Gilden
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
In vitro analyses of varicella zoster virus (VZV) reactivation from latency in human ganglia have been hampered by the inability to isolate virus by explantation or cocultivation techniques. Furthermore, attempts to study interaction of VZV with neurons in experimentally infected ganglion cells in vitro have been impaired by the presence of nonneuronal cells, which become productively infected and destroy the cultures. We have developed an in vitro model of VZV infection in which highly pure (>95 %) terminally differentiated human neurons derived from pluripotent stem cells were infected with VZV. At 2 weeks post-infection, infected neurons appeared healthy compared to VZV-infected human fetal lung fibroblasts (HFLs), which developed a cytopathic effect (CPE) within 1 week. Tissue culture medium from VZV-infected neurons did not produce a CPE in uninfected HFLs and did not contain PCR-amplifiable VZV DNA, but cocultivation of infected neurons with uninfected HFLs did produce a CPE. The nonproductively infected neurons contained multiple regions of the VZV genome, as well as transcripts and proteins corresponding to VZV immediate-early, early, and late genes. No markers of the apoptotic caspase cascade were detected in healthy-appearing VZV-infected neurons. VZV infection of highly pure terminally differentiated human neurons provides a unique in vitro system to study the VZV-neuronal relationship and the potential to investigate mechanisms of VZV reactivation.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of NeuroVirology
Numberingvol. 19, no. 1, pp. 75-81
p-ISSN1355-0284
e-ISSN1538-2443
DOIhttps://doi.org/10.1007/s13365-012-0142-x
URN:NBNurn:nbn:hr:184:750399
Publication2013-12-12
Publication2013-02
ProjectNumber: 229585
Title (english): The Center for Antibody Production Rijeka: Upgrading the Central Research and Service Infrastructure for the South Eastern Region of Europe
Acronym: CAPRI2010
Jurisdiction: eu
Funding stream: FP7
Document URLhttp://link.springer.com/10.1007/s13365-012-0142-x
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2018-10-30 13:18:59