prikaz prve stranice dokumenta Varicella zoster virus infection of highly pure terminally differentiated human neurons
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Varicella zoster virus infection of highly pure terminally differentiated human neurons
Journal of NeuroVirology, 19 (2013), 1; 75-81. https://doi.org/10.1007/s13365-012-0142-x
Yu, Xiaoli; Seitz, Scott; Pointon, Tiffany; Bowlin, Jacqueline L.; Cohrs, Randall J.; Jonjić, Stipan; Haas, Jürgen; Wellish, Mary; Gilden, Don

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

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Yu, X., Seitz, S., Pointon, T., Bowlin, J. L., Cohrs, R. J., Jonjić, S. ... Gilden, D. (2013). Varicella zoster virus infection of highly pure terminally differentiated human neurons. Journal of NeuroVirology, 19. (1), 75-81. doi: 10.1007/s13365-012-0142-x

Yu, Xiaoli, et al. "Varicella zoster virus infection of highly pure terminally differentiated human neurons." Journal of NeuroVirology, vol. 19, br. 1, 2013, str. 75-81. https://doi.org/10.1007/s13365-012-0142-x

Yu, Xiaoli, Scott Seitz, Tiffany Pointon, Jacqueline L. Bowlin, Randall J. Cohrs, Stipan Jonjić, Jürgen Haas, Mary Wellish i Don Gilden. "Varicella zoster virus infection of highly pure terminally differentiated human neurons." Journal of NeuroVirology 19, br. 1 (2013): 75-81. https://doi.org/10.1007/s13365-012-0142-x

Yu, X., et al. (2013) 'Varicella zoster virus infection of highly pure terminally differentiated human neurons', Journal of NeuroVirology, 19(1), str. 75-81. doi: 10.1007/s13365-012-0142-x

Yu X, Seitz S, Pointon T, Bowlin JL, Cohrs RJ, Jonjić S, i sur.. Varicella zoster virus infection of highly pure terminally differentiated human neurons. Journal of NeuroVirology [Internet]. 12.12.2013. [pristupljeno 15.05.2024.];19(1):75-81. doi: 10.1007/s13365-012-0142-x

X. Yu, et al., "Varicella zoster virus infection of highly pure terminally differentiated human neurons", Journal of NeuroVirology, vol. 19, br. 1, str. 75-81, Prosinac 2013. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:750399. [Citirano: 15.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:750399
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Naslov (engleski)Varicella zoster virus infection of highly pure terminally differentiated human neurons
AutorXiaoli Yu
AutorScott Seitz
AutorTiffany Pointon
AutorJacqueline L. Bowlin
AutorRandall J. Cohrs
AutorStipan Jonjić
AutorJürgen Haas
AutorMary Wellish
AutorDon Gilden
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
In vitro analyses of varicella zoster virus (VZV) reactivation from latency in human ganglia have been hampered by the inability to isolate virus by explantation or cocultivation techniques. Furthermore, attempts to study interaction of VZV with neurons in experimentally infected ganglion cells in vitro have been impaired by the presence of nonneuronal cells, which become productively infected and destroy the cultures. We have developed an in vitro model of VZV infection in which highly pure (>95 %) terminally differentiated human neurons derived from pluripotent stem cells were infected with VZV. At 2 weeks post-infection, infected neurons appeared healthy compared to VZV-infected human fetal lung fibroblasts (HFLs), which developed a cytopathic effect (CPE) within 1 week. Tissue culture medium from VZV-infected neurons did not produce a CPE in uninfected HFLs and did not contain PCR-amplifiable VZV DNA, but cocultivation of infected neurons with uninfected HFLs did produce a CPE. The nonproductively infected neurons contained multiple regions of the VZV genome, as well as transcripts and proteins corresponding to VZV immediate-early, early, and late genes. No markers of the apoptotic caspase cascade were detected in healthy-appearing VZV-infected neurons. VZV infection of highly pure terminally differentiated human neurons provides a unique in vitro system to study the VZV-neuronal relationship and the potential to investigate mechanisms of VZV reactivation.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of NeuroVirology
Brojčani podacivol. 19, br. 1, str. 75-81
p-ISSN1355-0284
e-ISSN1538-2443
DOIhttps://doi.org/10.1007/s13365-012-0142-x
URN:NBNurn:nbn:hr:184:750399
Datum objave publikacije2013-12-12
Datum objave publikacije2013-02
ProjektŠifra: 229585
Naziv (engleski): The Center for Antibody Production Rijeka: Upgrading the Central Research and Service Infrastructure for the South Eastern Region of Europe
Kratica: CAPRI2010
Pravna nadležnost: eu
Financijer: EC
Linija financiranja: FP7
URL dokumentahttp://link.springer.com/10.1007/s13365-012-0142-x
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2018-10-30 13:18:59