prikaz prve stranice dokumenta Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor
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Scientific paper - Original scientific paper
Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor
Journal of General Virology, 75 (1994); 101-110. urn:nbn:hr:184:602109
Lučin, Pero; Jonjić, Stipan; Messerle, Martin; Polić, Bojan; Hengel, Hartmut; Koszinowski, Ulrich H.

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Lučin, P., Jonjić, S., Messerle, M., Polić, B., Hengel, H. & Koszinowski, U. H. (1994). Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor. Journal of General Virology, 75., 101-110. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:602109

Lučin, Pero, et al. "Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor." Journal of General Virology, vol. 75, 1994, pp. 101-110. https://urn.nsk.hr/urn:nbn:hr:184:602109

Lučin, Pero, Stipan Jonjić, Martin Messerle, Bojan Polić, Hartmut Hengel and Ulrich H. Koszinowski. "Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor." Journal of General Virology 75 (1994): 101-110. https://urn.nsk.hr/urn:nbn:hr:184:602109

Lučin, P., et al. (1994) 'Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor', Journal of General Virology, 75, pp. 101-110. Available at: https://urn.nsk.hr/urn:nbn:hr:184:602109 (Accessed 05 May 2024)

Lučin P, Jonjić S, Messerle M, Polić B, Hengel H, Koszinowski UH. Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor. Journal of General Virology [Internet]. 1994 [cited 2024 May 05];75:101-110. Available at: https://urn.nsk.hr/urn:nbn:hr:184:602109

P. Lučin, S. Jonjić, M. Messerle, B. Polić, H. Hengel and U. H. Koszinowski, "Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor", Journal of General Virology, vol. 75, pp. 101-110, 1994. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:602109. [Accessed: 05 May 2024]

Metadata
Title (english)Late phase inhibition of murine cytomegalovirus replication by synergistic action of interferon-gamma and tumour necrosis factor
AuthorPero Lučin
AuthorStipan Jonjić
AuthorMartin Messerle
AuthorBojan Polić
AuthorHartmut Hengel
AuthorUlrich H. Koszinowski
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
We have shown previously that the antiviral function of CD4 + T lymphocytes against murine cytomegalovirus (MCMV) is associated with the release of interferon-?, (IFN-),). We now demonstrate that IFN-?, and tumour necrosis factor alpha (TNF-~<) display synergism in their antiviral activity. As little as 2 ng/ml of IFN-), and TNF-~ reduced the virus yield by about three orders of magnitude. There was no effect on immediate early (IE) and early (E) gene expression as far as the candidate genes IE1, E1 and those encoding the major DNAbinding protein and the DNA polymerase were concemed. Late gene transcription, assayed by the candidate genes encoding glycoprotein B and the MCMV homologue of ICP 18.5, was blocked and MCMV DNA replication was found to be reduced but not halted. The most prominent finding of the cytokine effect, seen by electron microscopy, was an alteration of nucleocapsid formation. Altogether, the synergism is multifaceted and acts at more than one stage during viral morphogenesis. Because the cytokines clearly do not act at an early stage of infection we conclude that the mode of cytokine activity differs between alpha- and betaherpesviruses.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of General Virology
Numberingvol. 75, pp. 101-110
p-ISSN0022-1317
e-ISSN1465-2099
URN:NBNurn:nbn:hr:184:602109
Publication1994
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2019-11-19 16:21:40