prikaz prve stranice dokumenta Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
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Scientific paper - Original scientific paper
Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
BioMed Research International, 2020 (2020); 1-7. https://doi.org/10.1155/2020/6826983
Kelava, Ina; Marečić, Valentina; Fućak, Petra; Ivek, Elena; Kolarić, Dominik; Ožanič, Mateja; Mihelčić, Mirna; Šantić, Marina

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Kelava, I., Marečić, V., Fućak, P., Ivek, E., Kolarić, D., Ožanič, M. ... Šantić, M. (2020). Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum. BioMed Research International, 2020., 1-7. doi: 10.1155/2020/6826983

Kelava, Ina, et al. "Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum." BioMed Research International, vol. 2020, 2020, pp. 1-7. https://doi.org/10.1155/2020/6826983

Kelava, Ina, Valentina Marečić, Petra Fućak, Elena Ivek, Dominik Kolarić, Mateja Ožanič, Mirna Mihelčić and Marina Šantić. "Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum." BioMed Research International 2020 (2020): 1-7. https://doi.org/10.1155/2020/6826983

Kelava, I., et al. (2020) 'Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum', BioMed Research International, 2020, pp. 1-7. doi: 10.1155/2020/6826983

Kelava I, Marečić V, Fućak P, Ivek E, Kolarić D, Ožanič M, and sur.. Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum. BioMed Research International [Internet]. 2020 January 22 [cited 2024 April 28];2020:1-7. doi: 10.1155/2020/6826983

I. Kelava, et al., "Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum", BioMed Research International, vol. 2020, pp. 1-7, January 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:036690. [Accessed: 28 April 2024]

Metadata
Title (english)Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
AuthorIna Kelava
AuthorValentina Marečić
AuthorPetra Fućak
AuthorElena Ivek
AuthorDominik Kolarić
AuthorMateja Ožanič
AuthorMirna Mihelčić
AuthorMarina Šantić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
The amoeba has been used as a model organism to study host-pathogen interaction in many intracellular bacteria. is a Gram-negative, highly infectious bacterium that causes the zoonotic disease tularemia. The bacterium is able to replicate in different phagocytic and nonphagocytic cells including mammalian, amoebae, and arthropod cells. The aim of this study was to determine the optimal temperature and infection dose in the interaction of with in order to establish a model of infection in the social amoeba. The amoeba cells were infected with a different multiplicity of infection (5, 10, and 100) and incubated at different temperatures (22, 25, 27, 30, and 37°C). The number of intracellular bacteria within , as well as cytotoxicity, was determined at 2, 4, 24, 48, and 72 hours after infection. Our results showed that the optimal temperature for intracellular replication within amoeba is 30°C with the MOI of 10. We can conclude that this MOI and temperature induced the optimal growth of bacteria in with low cytotoxicity.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleBioMed Research International
Numberingvol. 2020, pp. 1-7, 6826983
p-ISSN2314-6133
e-ISSN2314-6141
DOIhttps://doi.org/10.1155/2020/6826983
URN:NBNurn:nbn:hr:184:036690
Publication2020-01-22
ProjectNumber: uniri-biomed-18-128
Title (croatian): Francisella molekularni mehanizmi prilagodbe na stanice amebama
Title (english): Francisella-the molecular mechanisms of adaptation on amoeba cells
Leader: Marina Šantić
Jurisdiction: Croatia
Funding stream: Sveučilište u Rijeci
ProjectNumber: IP-2016-06-9003
Title (croatian): Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije
Title (english): The role of the intracellular life cycle of Francisella tularensis for the pathogenesis of experimental tularemia
Acronym: FRANCYCLE
Leader: Marina Šantić
Jurisdiction: Croatia
Funding stream: IP
Document URLhttps://www.hindawi.com/journals/bmri/2020/6826983/
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2020-03-28 16:02:44