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Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
BioMed Research International, 2020 (2020); 1-7. https://doi.org/10.1155/2020/6826983
Kelava, Ina; Marečić, Valentina; Fućak, Petra; Ivek, Elena; Kolarić, Dominik; Ožanič, Mateja; Mihelčić, Mirna; Šantić, Marina

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

Citirajte ovaj rad

Kelava, I., Marečić, V., Fućak, P., Ivek, E., Kolarić, D., Ožanič, M. ... Šantić, M. (2020). Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum. BioMed Research International, 2020., 1-7. doi: 10.1155/2020/6826983

Kelava, Ina, et al. "Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum." BioMed Research International, vol. 2020, 2020, str. 1-7. https://doi.org/10.1155/2020/6826983

Kelava, Ina, Valentina Marečić, Petra Fućak, Elena Ivek, Dominik Kolarić, Mateja Ožanič, Mirna Mihelčić i Marina Šantić. "Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum." BioMed Research International 2020 (2020): 1-7. https://doi.org/10.1155/2020/6826983

Kelava, I., et al. (2020) 'Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum', BioMed Research International, 2020, str. 1-7. doi: 10.1155/2020/6826983

Kelava I, Marečić V, Fućak P, Ivek E, Kolarić D, Ožanič M, i sur.. Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum. BioMed Research International [Internet]. 22.01.2020. [pristupljeno 12.05.2024.];2020:1-7. doi: 10.1155/2020/6826983

I. Kelava, et al., "Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum", BioMed Research International, vol. 2020, str. 1-7, Siječanj 2020. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:036690. [Citirano: 12.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:036690
Podaci o radu
Naslov (engleski)Optimisation of External Factors for the Growth of Francisella novicida within Dictyostelium discoideum
AutorIna Kelava
AutorValentina Marečić
AutorPetra Fućak
AutorElena Ivek
AutorDominik Kolarić
AutorMateja Ožanič
AutorMirna Mihelčić
AutorMarina Šantić
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za mikrobiologiju i parazitologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
The amoeba has been used as a model organism to study host-pathogen interaction in many intracellular bacteria. is a Gram-negative, highly infectious bacterium that causes the zoonotic disease tularemia. The bacterium is able to replicate in different phagocytic and nonphagocytic cells including mammalian, amoebae, and arthropod cells. The aim of this study was to determine the optimal temperature and infection dose in the interaction of with in order to establish a model of infection in the social amoeba. The amoeba cells were infected with a different multiplicity of infection (5, 10, and 100) and incubated at different temperatures (22, 25, 27, 30, and 37°C). The number of intracellular bacteria within , as well as cytotoxicity, was determined at 2, 4, 24, 48, and 72 hours after infection. Our results showed that the optimal temperature for intracellular replication within amoeba is 30°C with the MOI of 10. We can conclude that this MOI and temperature induced the optimal growth of bacteria in with low cytotoxicity.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBioMed Research International
Brojčani podacivol. 2020, str. 1-7, 6826983
p-ISSN2314-6133
e-ISSN2314-6141
DOIhttps://doi.org/10.1155/2020/6826983
URN:NBNurn:nbn:hr:184:036690
Datum objave publikacije2020-01-22
ProjektŠifra: uniri-biomed-18-128
Naziv (hrvatski): Francisella molekularni mehanizmi prilagodbe na stanice amebama
Naziv (engleski): Francisella-the molecular mechanisms of adaptation on amoeba cells
Voditelj: Marina Šantić
Pravna nadležnost: Hrvatska
Financijer: NadSve
Linija financiranja: Sveučilište u Rijeci
ProjektŠifra: IP-2016-06-9003
Naziv (hrvatski): Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije
Naziv (engleski): The role of the intracellular life cycle of Francisella tularensis for the pathogenesis of experimental tularemia
Kratica: FRANCYCLE
Voditelj: Marina Šantić
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
URL dokumentahttps://www.hindawi.com/journals/bmri/2020/6826983/
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2020-03-28 16:02:44