prikaz prve stranice dokumenta Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR
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Scientific paper - Original scientific paper
Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR
European Journal of Immunology, 43 (2013), 8; 2138-2150. https://doi.org/10.1002/eji.201243072
Stanietsky, Noa; Roviš, Tihana Lenac; Glasner, Ariella; Seidel, Einat; Tsukerman, Pinchas; Yamin, Rachel; Enk, Jonatan; Jonjić, Stipan; Mandelboim, Ofer

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Stanietsky, N., Roviš, T. L., Glasner, A., Seidel, E., Tsukerman, P., Yamin, R. ... Mandelboim, O. (2013). Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR. European Journal of Immunology, 43. (8), 2138-2150. doi: 10.1002/eji.201243072

Stanietsky, Noa, et al. "Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR." European Journal of Immunology, vol. 43, no. 8, 2013, pp. 2138-2150. https://doi.org/10.1002/eji.201243072

Stanietsky, Noa, Tihana Lenac Roviš, Ariella Glasner, Einat Seidel, Pinchas Tsukerman, Rachel Yamin, Jonatan Enk, Stipan Jonjić and Ofer Mandelboim. "Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR." European Journal of Immunology 43, no. 8 (2013): 2138-2150. https://doi.org/10.1002/eji.201243072

Stanietsky, N., et al. (2013) 'Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR', European Journal of Immunology, 43(8), pp. 2138-2150. doi: 10.1002/eji.201243072

Stanietsky N, Roviš TL, Glasner A, Seidel E, Tsukerman P, Yamin R, and sur.. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR. European Journal of Immunology [Internet]. 2013 August [cited 2024 October 06];43(8):2138-2150. doi: 10.1002/eji.201243072

N. Stanietsky, et al., "Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR", European Journal of Immunology, vol. 43, no. 8, pp. 2138-2150, August 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:164597. [Accessed: 06 October 2024]

Metadata
Title (english)Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR
AuthorNoa Stanietsky
AuthorTihana Lenac Roviš
AuthorAriella Glasner
AuthorEinat Seidel
AuthorPinchas Tsukerman
AuthorRachel Yamin
AuthorJonatan Enk
AuthorStipan Jonjić
AuthorOfer Mandelboim
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
The activity of natural killer (NK) cells is controlled by a balance of signals derived from inhibitory and activating receptors. TIGIT is a novel inhibitory receptor, recently shown in humans to interact with two ligands: PVR and Nectin2 and to inhibit human NK‐cell cytotoxicity. Whether mouse TIGIT (mTIGIT) inhibits mouse NK‐cell cytotoxicity is unknown. Here we show that mTIGIT is expressed by mouse NK cells and interacts with mouse PVR. Using mouse and human Ig fusion proteins we show that while the human TIGIT (hTIGIT) cross‐reacts with mouse PVR (mPVR), the binding of mTIGIT is restricted to mPVR. We further demonstrate using surface plasmon resonance (SPR) and staining with Ig fusion proteins that mTIGIT binds to mPVR with higher affinity than the co‐stimulatory PVR‐binding receptor mouse DNAM1 (mDNAM1). Functionally, we show that triggering of mTIGIT leads to the inhibition of NK‐cell cytotoxicity, that IFN‐γ secretion is enhanced when mTIGIT is blocked and that the TIGIT‐mediated inhibition is dominant over the signals delivered by the PVR‐binding co‐stimulatory receptors. Additionally, we identify the inhibitory motif responsible for mTIGIT inhibition. In conclusion, we show that TIGIT is a powerful inhibitory receptor for mouse NK cells.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleEuropean Journal of Immunology
Numberingvol. 43, no. 8, pp. 2138-2150
p-ISSN0014-2980
DOIhttps://doi.org/10.1002/eji.201243072
URN:NBNurn:nbn:hr:184:164597
Publication2013-08
ProjectNumber: 320473
Title (croatian): Recognition of bacteria by NK cells
Acronym: BACNK
Jurisdiction: eu
Funding stream: FP7
ProjectNumber: 1R01AI083201-01
Title (croatian): Viral evasion of NK cells
Jurisdiction: United States
Document URLhttp://doi.wiley.com/10.1002/eji.201243072
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) icon
Created on2020-04-19 13:39:47