prikaz prve stranice dokumenta Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells
  Download
PDF 2.08 MB
Scientific paper - Original scientific paper
Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells
International Journal of Molecular Sciences, 22 (2021), 9; 4399. https://doi.org/10.3390/ijms22094399
Rački, Valentino; Marcelic, Marina; Štimac, Igor; Petrić, Daniela; Kučić, Natalia

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Rački, V., Marcelic, M., Štimac, I., Petrić, D. & Kučić, N. (2021). Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells. International Journal of Molecular Sciences, 22. (9). doi: 10.3390/ijms22094399

Rački, Valentino, et al. "Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells." International Journal of Molecular Sciences, vol. 22, no. 9, 2021. https://doi.org/10.3390/ijms22094399

Rački, Valentino, Marina Marcelic, Igor Štimac, Daniela Petrić and Natalia Kučić. "Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells." International Journal of Molecular Sciences 22, no. 9 (2021). https://doi.org/10.3390/ijms22094399

Rački, V., et al. (2021) 'Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells', International Journal of Molecular Sciences, 22(9). doi: 10.3390/ijms22094399

Rački V, Marcelic M, Štimac I, Petrić D, Kučić N. Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells. International Journal of Molecular Sciences [Internet]. 2021 April 22 [cited 2024 November 23];22(9). doi: 10.3390/ijms22094399

V. Rački, M. Marcelic, I. Štimac, D. Petrić and N. Kučić, "Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells", International Journal of Molecular Sciences, vol. 22, no. 9, April 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:035173. [Accessed: 23 November 2024]

Metadata
Title (english)Effects of Haloperidol, Risperidone, and Aripiprazole on the Immunometabolic Properties of BV-2 Microglial Cells
AuthorValentino Rački
AuthorMarina Marcelic
AuthorIgor Štimac
AuthorDaniela Petrić
AuthorNatalia Kučić
MBZ: 239812
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Neurology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Psychiatry and Psychological Medicine)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Neurology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Psychiatry
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Abstract (english)
Microglial cells are resident macrophages in the brain that have been implicated in the pathophysiology of schizophrenia. There is a lack of studies covering the effects of antipsychotics on microglial cells. The current literature points to a possible anti-inflammatory action without clear mechanisms of action. The aim of this study is to characterize the effects of haloperidol, risperidone and aripiprazole on BV-2 microglial cells in in vitro conditions. We have used immunofluorescence and flow cytometry to analyze the classical pro and anti-inflammatory markers, while a real-time metabolic assay (Seahorse) was used to assess metabolic function. We analyzed the expression of p70S6K to evaluate the mTOR pathway activity with Western blot. In this study, we demonstrate the varying effects of haloperidol, risperidone and aripiprazole administration in BV-2 microglial cells. All three tested antipsychotics were successful in reducing the pro-inflammatory action of microglial cells, although only aripiprazole increased the expression of anti-inflammatory markers. Most significant differences in the possible mechanisms of action were seen in the real-time metabolic assays and in the mTORC1 signaling pathway activity, with aripiprazole being the only antipsychotic to reduce the mTORC1 activity. Our results shed some new light on the effects of haloperidol, risperidone and aripiprazole action in microglial cells, and reveal a novel possible mechanism of action for aripiprazole.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 22, no. 9, 4399
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms22094399
URN:NBNurn:nbn:hr:184:035173
Publication2021-04-22
ProjectNumber: 13.06.1.3.45,
Title (croatian): Endocitoza MHC molekula I razreda u mikroglijalnim stanicama inficiranih citomegalovirusom
Leader: Natalija Kučić
Jurisdiction: Croatia
Document URLhttps://www.mdpi.com/1422-0067/22/9/4399
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-05-13 20:13:02