prikaz prve stranice dokumenta Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness
  Download
PDF 23.4 MB
Scientific paper - Original scientific paper
Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness
International Journal of Molecular Sciences, 22 (2021), 16; 9095. https://doi.org/10.3390/ijms22169095
Mušković, Martina; Ćavar, Iva; Lesar, Andrija; Lončarić, Martin; Malatesti, Nela; Gobin, Ivana

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Mušković, M., Ćavar, I., Lesar, A., Lončarić, M., Malatesti, N. & Gobin, I. (2021). Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness. International Journal of Molecular Sciences, 22. (16). doi: 10.3390/ijms22169095

Mušković, Martina, et al. "Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness." International Journal of Molecular Sciences, vol. 22, no. 16, 2021. https://doi.org/10.3390/ijms22169095

Mušković, Martina, Iva Ćavar, Andrija Lesar, Martin Lončarić, Nela Malatesti and Ivana Gobin. "Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness." International Journal of Molecular Sciences 22, no. 16 (2021). https://doi.org/10.3390/ijms22169095

Mušković, M., et al. (2021) 'Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness', International Journal of Molecular Sciences, 22(16). doi: 10.3390/ijms22169095

Mušković M, Ćavar I, Lesar A, Lončarić M, Malatesti N, Gobin I. Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness. International Journal of Molecular Sciences [Internet]. 2021 August 23 [cited 2024 May 03];22(16). doi: 10.3390/ijms22169095

M. Mušković, I. Ćavar, A. Lesar, M. Lončarić, N. Malatesti and I. Gobin, "Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness", International Journal of Molecular Sciences, vol. 22, no. 16, August 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:159718. [Accessed: 03 May 2024]

Metadata
Title (english)Photodynamic Inactivation of Legionella pneumophila Biofilm Formation by Cationic Tetra- and Tripyridylporphyrins in Waters of Different Hardness
AuthorMartina Mušković
AuthorIva Ćavar
AuthorAndrija Lesar
AuthorMartin Lončarić
AuthorNela Malatesti
AuthorIvana Gobin
Author's institutionUniversity of Rijeka
(Faculty of Biotechnology and Drug Development)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract (english)
The bacterium Legionella pneumophila is still one of the probable causes of waterborne diseases, causing serious respiratory illnesses. In the aquatic systems, L. pneumophila exists inside free-living amoebae or can form biofilms. Currently developed disinfection methods are not sufficient for complete eradication of L. pneumophila biofilms in water systems of interest. Photodynamic inactivation (PDI) is a method that results in an antimicrobial effect by using a combination of light and a photosensitizer (PS). In this work, the effect of PDI in waters of natural origin and of different hardness, as a treatment against L. pneumophila biofilm, was investigated. Three cationic tripyridylporphyrins, which were previously described as efficient agents against L. pneumophila alone, were used as PSs. We studied how differences in water hardness affect the PSs’ stability, the production of singlet oxygen, and the PDI activity on L. pneumophila adhesion and biofilm formation and in biofilm destruction. Amphiphilic porphyrin showed a stronger tendency for aggregation in hard and soft water, but its production of singlet oxygen was higher in comparison to tri- and tetracationic hydrophilic porphyrins that were stable in all water samples. All three studied porphyrins were shown to be effective as PDI agents against the adhesion of the L. pneumophila to polystyrene, against biofilm formation, and in the destruction of the formed biofilm, in their micromolar concentrations. However, a higher number of dissolved ions, i.e., water hardness, generally reduced somewhat the PDI activity of all the porphyrins at all tested biofilm growth stages.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 22, no. 16, 9095
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms22169095
URN:NBNurn:nbn:hr:184:159718
Publication2021-08-23
Document URLhttps://www.mdpi.com/1422-0067/22/16/9095
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-09-03 09:22:13