prikaz prve stranice dokumenta Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of
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Scientific paper - Original scientific paper
Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of
Infection and Immunity, 78 (2010), 3; 1123-1134. https://doi.org/10.1128/IAI.00913-09
Habyarimana, Fabien; Price, Chris T.; Šantić, Marina; Al-Khodor, Souhaila; Kwaik, Yousef Abu

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Habyarimana, F., Price, C. T., Šantić, M., Al Khodor, S. & Kwaik, Y. A. (2010). Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of. Infection and Immunity, 78. (3), 1123-1134. doi: 10.1128/IAI.00913-09

Habyarimana, Fabien, et al. "Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of." Infection and Immunity, vol. 78, no. 3, 2010, pp. 1123-1134. https://doi.org/10.1128/IAI.00913-09

Habyarimana, Fabien, Chris T. Price, Marina Šantić, Souhaila Al Khodor and Yousef Abu Kwaik. "Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of." Infection and Immunity 78, no. 3 (2010): 1123-1134. https://doi.org/10.1128/IAI.00913-09

Habyarimana, F., et al. (2010) 'Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of', Infection and Immunity, 78(3), pp. 1123-1134. doi: 10.1128/IAI.00913-09

Habyarimana F, Price CT, Šantić M, Al Khodor S, Kwaik YA. Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of. Infection and Immunity [Internet]. 2010 March [cited 2024 August 30];78(3):1123-1134. doi: 10.1128/IAI.00913-09

F. Habyarimana, C. T. Price, M. Šantić, S. Al Khodor and Y. A. Kwaik, "Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of", Infection and Immunity, vol. 78, no. 3, pp. 1123-1134, March 2010. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:239208. [Accessed: 30 August 2024]

Metadata
Title (english)Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of
AuthorFabien Habyarimana
AuthorChris T. Price
AuthorMarina Šantić
AuthorSouhaila Al-Khodor
AuthorYousef Abu Kwaik
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
ABSTRACT Although most Dot/Icm-translocated effectors of are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors, AnkH and AnkJ are required for intracellular proliferation. In this report, we show that the IcmSW chaperones are essential for translocation of AnkJ but not AnkH. The 10 C-terminal residues and the ANK domains of AnkH and AnkJ are required for translocation. Our data indicate that the two ANK domains of AnkH are critical domains required for the function of the effector in intracellular replication of . The and mutants are severely defective in intrapulmonary proliferation in mice. Expression of AnkH and AnkJ fusions within HEK293 cells show a punctuate distribution in the cytosol but no association with endocytic vesicles, the Golgi apparatus or the endoplasmic reticulum. Interestingly, the defect in intracellular proliferation of the or mutants is rescued in HEK293 cells expressing the respective protein. We conclude that AnkH and AnkJ are effectors translocated by the Dot/Icm system by distinct mechanisms and modulate distinct cytosolic processes in the host cell.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleInfection and Immunity
Numberingvol. 78, no. 3, pp. 1123-1134
p-ISSN0019-9567
e-ISSN1098-5522
DOIhttps://doi.org/10.1128/IAI.00913-09
URN:NBNurn:nbn:hr:184:239208
Publication2010-03
Document URLhttps://journals.asm.org/doi/10.1128/IAI.00913-09
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-01-28 09:26:38