prikaz prve stranice dokumenta Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila
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Scientific paper - Original scientific paper
Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila
Journal of Experimental Medicine, 207 (2010), 8; 1713-1726. https://doi.org/10.1084/jem.20100771
Price, Christopher T.D.; Al-Quadan, Tasneem; Šantić, Marina; Jones, Snake C.; Abu Kwaik, Yousef

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Price, C. T., Al Quadan, T., Šantić, M., Jones, S. C. & Abu Kwaik, Y. (2010). Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila. Journal of Experimental Medicine, 207. (8), 1713-1726. doi: 10.1084/jem.20100771

Price, Christopher T.D., et al. "Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila." Journal of Experimental Medicine, vol. 207, no. 8, 2010, pp. 1713-1726. https://doi.org/10.1084/jem.20100771

Price, Christopher T.D., Tasneem Al Quadan, Marina Šantić, Snake C. Jones and Yousef Abu Kwaik. "Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila." Journal of Experimental Medicine 207, no. 8 (2010): 1713-1726. https://doi.org/10.1084/jem.20100771

Price, C. T., et al. (2010) 'Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila', Journal of Experimental Medicine, 207(8), pp. 1713-1726. doi: 10.1084/jem.20100771

Price CT, Al Quadan T, Šantić M, Jones SC, Abu Kwaik Y. Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila. Journal of Experimental Medicine [Internet]. 2010 August 02 [cited 2024 August 30];207(8):1713-1726. doi: 10.1084/jem.20100771

C. T. Price, T. Al Quadan, M. Šantić, S. C. Jones and Y. Abu Kwaik, "Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila", Journal of Experimental Medicine, vol. 207, no. 8, pp. 1713-1726, August 2010. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:142635. [Accessed: 30 August 2024]

Metadata
Title (english)Exploitation of conserved eukaryotic host cell farnesylation machinery by an F-box effector of Legionella pneumophila
AuthorChristopher T.D. Price
AuthorTasneem Al-Quadan
AuthorMarina Šantić
AuthorSnake C. Jones
AuthorYousef Abu Kwaik
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Farnesylation involves covalent linkage of eukaryotic proteins to a lipid moiety to anchor them into membranes, which is essential for the biological function of Ras and other proteins. A large cadre of bacterial effectors is injected into host cells by intravacuolar pathogens through elaborate type III–VII translocation machineries, and many of these effectors are incorporated into the pathogen-containing vacuolar membrane by unknown mechanisms. The Dot/Icm type IV secretion system of Legionella pneumophila injects into host cells the F-box effector Ankyrin B (AnkB), which functions as platforms for the docking of polyubiquitinated proteins to the Legionella-containing vacuole (LCV) to enable intravacuolar proliferation in macrophages and amoeba. We show that farnesylation of AnkB is indispensable for its anchoring to the cytosolic face of the LCV membrane, for its biological function within macrophages and Dictyostelium discoideum, and for intrapulmonary proliferation in mice. Remarkably, the protein farnesyltransferase, RCE-1 (Ras-converting enzyme-1), and isoprenyl cysteine carboxyl methyltransferase host farnesylation enzymes are recruited to the LCV in a Dot/Icm-dependent manner and are essential for the biological function of AnkB. In conclusion, this study shows novel localized recruitment of the host farnesylation machinery and its anchoring of an F-box effector to the LCV membrane, and this is essential for biological function in vitro and in vivo.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Experimental Medicine
Numberingvol. 207, no. 8, pp. 1713-1726
p-ISSN0022-1007
e-ISSN1540-9538
DOIhttps://doi.org/10.1084/jem.20100771
URN:NBNurn:nbn:hr:184:142635
Publication2010-08-02
Document URLhttps://rupress.org/jem/article/207/8/1713/46048/Exploitation-of-conserved-eukaryotic-host-cell
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-01-28 09:44:47