prikaz prve stranice dokumenta Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae
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Scientific paper - Original scientific paper
Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae
Environmental Toxicology, 35 (2020), 3; 346-358. https://doi.org/10.1002/tox.22870
Justinić, Iva; Katić, Ana; Ćurko-Cofek, Božena; Blagović, Branka; Čanadi Jurešić, Gordana

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Justinić, I., Katić, A., Ćurko Cofek, B., Blagović, B. & Čanadi Jurešić, G. (2020). Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae. Environmental Toxicology, 35. (3), 346-358. doi: 10.1002/tox.22870

Justinić, Iva, et al. "Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae." Environmental Toxicology, vol. 35, no. 3, 2020, pp. 346-358. https://doi.org/10.1002/tox.22870

Justinić, Iva, Ana Katić, Božena Ćurko Cofek, Branka Blagović and Gordana Čanadi Jurešić. "Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae." Environmental Toxicology 35, no. 3 (2020): 346-358. https://doi.org/10.1002/tox.22870

Justinić, I., et al. (2020) 'Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae', Environmental Toxicology, 35(3), pp. 346-358. doi: 10.1002/tox.22870

Justinić I, Katić A, Ćurko Cofek B, Blagović B, Čanadi Jurešić G. Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae. Environmental Toxicology [Internet]. 2020 [cited 2024 May 08];35(3):346-358. doi: 10.1002/tox.22870

I. Justinić, A. Katić, B. Ćurko Cofek, B. Blagović and G. Čanadi Jurešić, "Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae", Environmental Toxicology, vol. 35, no. 3, pp. 346-358, 2020. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:087331. [Accessed: 08 May 2024]

Metadata
Title (english)Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae
AuthorIva Justinić
AuthorAna Katić
AuthorBožena Ćurko-Cofek
AuthorBranka Blagović
AuthorGordana Čanadi Jurešić
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Chemistry and Biochemistry)
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Abstract (english)
The yeast Saccharomyces cerevisiae is a useful model for studying the influence of different stress factors on eukaryotic cells. In this work we used the pesticide imidacloprid, in the Confidor® formulation, as the stress factor and analyzed its influence on the metabolic activity, proteome and lipid content and composition of Saccharomyces cerevisiae yeast. During the cultivation of yeast, the lowest recommended application dose of Confidor® (0.025 %, v/v) was added to the growth media and its influence on the mitochondria, cytosol with microsomes and the whole yeast cells was monitored. The results show that under the stress provoked by the toxic effects of Confidor®, yeast cells density significantly decreased and the percentage of metabolically disturbed cells significantly increased comparing with untreated control. Also, there was a downregulation of majority of glycolytic, gluconeogenesis and TCA cycle enzymes (Fba1, Adh1, Hxk2, Tal1, Tdh1, Tdh3, Eno1) thus providing enough acetyl-CoA for the lipid restructuring and accumulation mechanism since we have found the changes in the cell and mitochondrial lipid content and FA composition. This data suggest that lipids could be the molecules that orchestrate the answer of the cells in the stress response to the Confidor® treatment.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleEnvironmental Toxicology
Numberingvol. 35, no. 3, pp. 346-358
p-ISSN1520-4081
e-ISSN1522-7278
DOIhttps://doi.org/10.1002/tox.22870
URN:NBNurn:nbn:hr:184:087331
Publication2020
Document URLhttp://bib.irb.hr/1028361
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2022-02-11 17:47:57