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Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae
Environmental Toxicology, 35 (2020), 3; 346-358. https://doi.org/10.1002/tox.22870
Justinić, Iva; Katić, Ana; Ćurko-Cofek, Božena; Blagović, Branka; Čanadi Jurešić, Gordana

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

Citirajte ovaj rad

Justinić, I., Katić, A., Ćurko Cofek, B., Blagović, B. i Čanadi Jurešić, G. (2020). Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae. Environmental Toxicology, 35. (3), 346-358. doi: 10.1002/tox.22870

Justinić, Iva, et al. "Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae." Environmental Toxicology, vol. 35, br. 3, 2020, str. 346-358. https://doi.org/10.1002/tox.22870

Justinić, Iva, Ana Katić, Božena Ćurko Cofek, Branka Blagović i Gordana Čanadi Jurešić. "Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae." Environmental Toxicology 35, br. 3 (2020): 346-358. https://doi.org/10.1002/tox.22870

Justinić, I., et al. (2020) 'Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae', Environmental Toxicology, 35(3), str. 346-358. doi: 10.1002/tox.22870

Justinić I, Katić A, Ćurko Cofek B, Blagović B, Čanadi Jurešić G. Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae. Environmental Toxicology [Internet]. 2020. [pristupljeno 23.11.2024.];35(3):346-358. doi: 10.1002/tox.22870

I. Justinić, A. Katić, B. Ćurko Cofek, B. Blagović i G. Čanadi Jurešić, "Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae", Environmental Toxicology, vol. 35, br. 3, str. 346-358, 2020. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:087331. [Citirano: 23.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:087331
Podaci o radu
Naslov (engleski)Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae
AutorIva Justinić
AutorAna Katić
AutorBožena Ćurko-Cofek
AutorBranka Blagović
AutorGordana Čanadi Jurešić
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za medicinsku kemiju, biokemiju i kliničku kemiju)
Znanstveno / umjetničko
područje, polje i grana		INTERDISCIPLINARNA PODRUČJA ZNANOSTI
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Sažetak (engleski)
The yeast Saccharomyces cerevisiae is a useful model for studying the influence of different stress factors on eukaryotic cells. In this work we used the pesticide imidacloprid, in the Confidor® formulation, as the stress factor and analyzed its influence on the metabolic activity, proteome and lipid content and composition of Saccharomyces cerevisiae yeast. During the cultivation of yeast, the lowest recommended application dose of Confidor® (0.025 %, v/v) was added to the growth media and its influence on the mitochondria, cytosol with microsomes and the whole yeast cells was monitored. The results show that under the stress provoked by the toxic effects of Confidor®, yeast cells density significantly decreased and the percentage of metabolically disturbed cells significantly increased comparing with untreated control. Also, there was a downregulation of majority of glycolytic, gluconeogenesis and TCA cycle enzymes (Fba1, Adh1, Hxk2, Tal1, Tdh1, Tdh3, Eno1) thus providing enough acetyl-CoA for the lipid restructuring and accumulation mechanism since we have found the changes in the cell and mitochondrial lipid content and FA composition. This data suggest that lipids could be the molecules that orchestrate the answer of the cells in the stress response to the Confidor® treatment.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaEnvironmental Toxicology
Brojčani podacivol. 35, br. 3, str. 346-358
p-ISSN1520-4081
e-ISSN1522-7278
DOIhttps://doi.org/10.1002/tox.22870
URN:NBNurn:nbn:hr:184:087331
Datum objave publikacije2020
URL dokumentahttp://bib.irb.hr/1028361
Vrsta resursaTekst
Prava pristupaOgraničen pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2022-02-11 17:47:57