prikaz prve stranice dokumenta Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury
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Scientific paper - Original scientific paper
Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury
Journal of biological regulators & homeostatic agents, 32 (2018), 4; 803-813. urn:nbn:hr:184:373424
Bobinac, Mirna; Ćelić, Tanja; Vukelić, Iva; Španjol, Josip; Rubinić, Nino; Bobinac, Dragica

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Bobinac, M., Ćelić, T., Vukelić, I., Španjol, J., Rubinić, N. & Bobinac, D. (2018). Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury. Journal of biological regulators & homeostatic agents, 32. (4), 803-813. Retrieved from https://urn.nsk.hr/urn:nbn:hr:184:373424

Bobinac, Mirna, et al. "Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury." Journal of biological regulators & homeostatic agents, vol. 32, no. 4, 2018, pp. 803-813. https://urn.nsk.hr/urn:nbn:hr:184:373424

Bobinac, Mirna, Tanja Ćelić, Iva Vukelić, Josip Španjol, Nino Rubinić and Dragica Bobinac. "Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury." Journal of biological regulators & homeostatic agents 32, no. 4 (2018): 803-813. https://urn.nsk.hr/urn:nbn:hr:184:373424

Bobinac, M., et al. (2018) 'Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury', Journal of biological regulators & homeostatic agents, 32(4), pp. 803-813. Available at: https://urn.nsk.hr/urn:nbn:hr:184:373424 (Accessed 25 December 2024)

Bobinac M, Ćelić T, Vukelić I, Španjol J, Rubinić N, Bobinac D. Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury. Journal of biological regulators & homeostatic agents [Internet]. 2018 [cited 2024 December 25];32(4):803-813. Available at: https://urn.nsk.hr/urn:nbn:hr:184:373424

M. Bobinac, T. Ćelić, I. Vukelić, J. Španjol, N. Rubinić and D. Bobinac, "Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury", Journal of biological regulators & homeostatic agents, vol. 32, no. 4, pp. 803-813, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:373424. [Accessed: 25 December 2024]

Metadata
Title (english)Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury
AuthorMirna Bobinac
AuthorTanja Ćelić
AuthorIva Vukelić
AuthorJosip Španjol
AuthorNino Rubinić
AuthorDragica Bobinac
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Anatomy)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Urology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Spinal cord ischemia-reperfusion injury (IRI) results in overproduction of reactive oxygen species leading to tissue oxidative stress which impacts the neuronal network in the spinal cord as well as glial cells. We investigated the expression of Nuclear factor erythroid 2-related factor 2 (Nrf2) in neurons and glial cells after occlusion of the abdominal aorta followed by IRI as well as the time-dependent expression of Nrf2 in the same cells. The experimental method of transient aortic occlusion was carried out on rats by cross-clamping of the abdominal aorta for 45 minutes. The animals used for this study were sacrificed 1 h, 6 h, and 48 h after reperfusion to determine time-related changes of Nrf2 expression, as well as changes of astrocyte activity in the spinal cord. Immunofluorescence results showed an increase in the staining intensity of Nrf2 expression in the neurons following ischemia with highest intensity 48 h post-reperfusion and an increase in a number of reactive astrocytes. Western blot analysis showed that Nrf2 protein expression increased in a cytoplasmic and nuclear fraction as early as 1 h after reperfusion and remained active 48 h after, resulting in increased expression of the main Nrf2 target gene HO-1. In conclusion, substances that enhance expression of Nrf2 may have the potential to prevent cellular damage to the spinal cord caused by IRI.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionAccepted version
Journal titleJournal of biological regulators & homeostatic agents
Numberingvol. 32, no. 4, pp. 803-813
p-ISSN0393-974X
URN:NBNurn:nbn:hr:184:373424
Publication2018
Document URLhttp://bib.irb.hr/953698
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2022-02-28 17:01:07