prikaz prve stranice dokumenta Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury
  Preuzmi
PDF 6.19 MB
Znanstveni rad - Izvorni znanstveni rad
Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury
Journal of biological regulators & homeostatic agents, 32 (2018), 4; 803-813. urn:nbn:hr:184:373424
Bobinac, Mirna; Ćelić, Tanja; Vukelić, Iva; Španjol, Josip; Rubinić, Nino; Bobinac, Dragica

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

Citirajte ovaj rad

Bobinac, M., Ćelić, T., Vukelić, I., Španjol, J., Rubinić, N. i Bobinac, D. (2018). Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury. Journal of biological regulators & homeostatic agents, 32. (4), 803-813. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:373424

Bobinac, Mirna, et al. "Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury." Journal of biological regulators & homeostatic agents, vol. 32, br. 4, 2018, str. 803-813. https://urn.nsk.hr/urn:nbn:hr:184:373424

Bobinac, Mirna, Tanja Ćelić, Iva Vukelić, Josip Španjol, Nino Rubinić i Dragica Bobinac. "Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury." Journal of biological regulators & homeostatic agents 32, br. 4 (2018): 803-813. https://urn.nsk.hr/urn:nbn:hr:184:373424

Bobinac, M., et al. (2018) 'Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury', Journal of biological regulators & homeostatic agents, 32(4), str. 803-813. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:373424 (Datum pristupa: 21.05.2024.)

Bobinac M, Ćelić T, Vukelić I, Španjol J, Rubinić N, Bobinac D. Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury. Journal of biological regulators & homeostatic agents [Internet]. 2018. [pristupljeno 21.05.2024.];32(4):803-813. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:373424

M. Bobinac, T. Ćelić, I. Vukelić, J. Španjol, N. Rubinić i D. Bobinac, "Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury", Journal of biological regulators & homeostatic agents, vol. 32, br. 4, str. 803-813, 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:373424. [Citirano: 21.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:373424
Podaci o radu
Naslov (engleski)Nuclear factor erythroid 2-related factor 2 and choline acetyltransferase co-expression in rat spinal cord neurons after ischemia-reperfusion injury
AutorMirna Bobinac
AutorTanja Ćelić
AutorIva Vukelić
AutorJosip Španjol
AutorNino Rubinić
AutorDragica Bobinac
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za anatomiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za urologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Spinal cord ischemia-reperfusion injury (IRI) results in overproduction of reactive oxygen species leading to tissue oxidative stress which impacts the neuronal network in the spinal cord as well as glial cells. We investigated the expression of Nuclear factor erythroid 2-related factor 2 (Nrf2) in neurons and glial cells after occlusion of the abdominal aorta followed by IRI as well as the time-dependent expression of Nrf2 in the same cells. The experimental method of transient aortic occlusion was carried out on rats by cross-clamping of the abdominal aorta for 45 minutes. The animals used for this study were sacrificed 1 h, 6 h, and 48 h after reperfusion to determine time-related changes of Nrf2 expression, as well as changes of astrocyte activity in the spinal cord. Immunofluorescence results showed an increase in the staining intensity of Nrf2 expression in the neurons following ischemia with highest intensity 48 h post-reperfusion and an increase in a number of reactive astrocytes. Western blot analysis showed that Nrf2 protein expression increased in a cytoplasmic and nuclear fraction as early as 1 h after reperfusion and remained active 48 h after, resulting in increased expression of the main Nrf2 target gene HO-1. In conclusion, substances that enhance expression of Nrf2 may have the potential to prevent cellular damage to the spinal cord caused by IRI.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaJournal of biological regulators & homeostatic agents
Brojčani podacivol. 32, br. 4, str. 803-813
p-ISSN0393-974X
URN:NBNurn:nbn:hr:184:373424
Datum objave publikacije2018
URL dokumentahttp://bib.irb.hr/953698
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2022-02-28 17:01:07