prikaz prve stranice dokumenta Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis
  Download
PDF 2.16 MB
Scientific paper - Original scientific paper
Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis
Current Issues in Molecular Biology, 44 (2022), 8; 3465-3480. https://doi.org/10.3390/cimb44080239
Omanovicć Kolarić, Tea; Kizivat, Tomislav; Mihaljević, Vjera; Zjalić, Milorad; Bilić Ćurčić, Ines; Kuna, Lucija; Smolić, Robert; Včev, Aleksandar; Wu, George Y.; Smolić, Martina

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Omanovicć Kolarić, T., Kizivat, T., Mihaljević, V., Zjalić, M., Bilić Ćurčić, I., Kuna, L. ... Smolić, M. (2022). Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis. Current Issues in Molecular Biology, 44. (8), 3465-3480. doi: 10.3390/cimb44080239

Omanovicć Kolarić, Tea, et al. "Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis." Current Issues in Molecular Biology, vol. 44, no. 8, 2022, pp. 3465-3480. https://doi.org/10.3390/cimb44080239

Omanovicć Kolarić, Tea, Tomislav Kizivat, Vjera Mihaljević, Milorad Zjalić, Ines Bilić Ćurčić, Lucija Kuna, Robert Smolić, Aleksandar Včev, George Y. Wu and Martina Smolić. "Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis." Current Issues in Molecular Biology 44, no. 8 (2022): 3465-3480. https://doi.org/10.3390/cimb44080239

Omanovicć Kolarić, T., et al. (2022) 'Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis', Current Issues in Molecular Biology, 44(8), pp. 3465-3480. doi: 10.3390/cimb44080239

Omanovicć Kolarić T, Kizivat T, Mihaljević V, Zjalić M, Bilić Ćurčić I, Kuna L, and sur.. Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis. Current Issues in Molecular Biology [Internet]. 2022 August 02 [cited 2024 June 23];44(8):3465-3480. doi: 10.3390/cimb44080239

T. Omanovicć Kolarić, et al., "Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis", Current Issues in Molecular Biology, vol. 44, no. 8, pp. 3465-3480, August 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:850995. [Accessed: 23 June 2024]

Metadata
Title (english)Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis
AuthorTea Omanovicć Kolarić
AuthorTomislav Kizivat
AuthorVjera Mihaljević
AuthorMilorad Zjalić
AuthorInes Bilić Ćurčić
AuthorLucija Kuna
AuthorRobert Smolić
AuthorAleksandar Včev
AuthorGeorge Y. Wu
AuthorMartina Smolić
Author's institutionUniversity of Rijeka
Faculty of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
(1) Background: With the aging of the population and polypharmacy encountered in the elderly, drug-induced steatosis (DIS) has become frequent cause of non-alcoholic steatosis (NAS). Indeed, NAS and DIS may co-exist, making the ability to distinguish between the entities ever more important. The aim of our study was to study cell culture models of NAS and DIS and determine the effects of liraglutide (LIRA) in those models. (2) Methods: Huh7 cells were treated with oleic acid (OA), or amiodarone (AMD) to establish models of NAS and DIS, respectively. Cells were treated with LIRA and cell viability was assessed by MTT, lipid accumulation by Oil-Red-O staining and triglyceride assay, and intracellular signals involved in hepatosteatosis were quantitated by RT-PCR. (3) Results: After exposure to various OA and AMD concentrations, those that achieved 80% of cells viabilities were used in further experiments to establish NAS and DIS models using 0.5 mM OA and 20 µM AMD, respectively. In both models, LIRA increased cell viability (p < 0.01). Lipid accumulation was increased in both models, with microsteatotic pattern in DIS, and macrosteatotic pattern in NAS which corresponds to greater triglyceride accumulation in latter. LIRA ameliorated these changes (p < 0.001), and downregulated expression of lipogenic ACSL1, PPARγ, and SREBP-1c pathways in the liver (p < 0.01) (4) Conclusions: LIRA ameliorates hepatocyte steatosis in Huh7 cell culture models of NAS and DIS.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCurrent Issues in Molecular Biology
Numberingvol. 44, no. 8, pp. 3465-3480
e-ISSN1467-3045
DOIhttps://doi.org/10.3390/cimb44080239
URN:NBNurn:nbn:hr:184:850995
Publication2022-08-02
Document URLhttps://www.mdpi.com/1467-3045/44/8/239
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-08-12 11:24:39