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Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis
Current Issues in Molecular Biology, 44 (2022), 8; 3465-3480. https://doi.org/10.3390/cimb44080239
Omanovicć Kolarić, Tea; Kizivat, Tomislav; Mihaljević, Vjera; Zjalić, Milorad; Bilić Ćurčić, Ines; Kuna, Lucija; Smolić, Robert; Včev, Aleksandar; Wu, George Y.; Smolić, Martina

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

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Omanovicć Kolarić, T., Kizivat, T., Mihaljević, V., Zjalić, M., Bilić Ćurčić, I., Kuna, L. ... Smolić, M. (2022). Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis. Current Issues in Molecular Biology, 44. (8), 3465-3480. doi: 10.3390/cimb44080239

Omanovicć Kolarić, Tea, et al. "Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis." Current Issues in Molecular Biology, vol. 44, br. 8, 2022, str. 3465-3480. https://doi.org/10.3390/cimb44080239

Omanovicć Kolarić, Tea, Tomislav Kizivat, Vjera Mihaljević, Milorad Zjalić, Ines Bilić Ćurčić, Lucija Kuna, Robert Smolić, Aleksandar Včev, George Y. Wu i Martina Smolić. "Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis." Current Issues in Molecular Biology 44, br. 8 (2022): 3465-3480. https://doi.org/10.3390/cimb44080239

Omanovicć Kolarić, T., et al. (2022) 'Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis', Current Issues in Molecular Biology, 44(8), str. 3465-3480. doi: 10.3390/cimb44080239

Omanovicć Kolarić T, Kizivat T, Mihaljević V, Zjalić M, Bilić Ćurčić I, Kuna L, i sur.. Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis. Current Issues in Molecular Biology [Internet]. 02.08.2022. [pristupljeno 23.06.2024.];44(8):3465-3480. doi: 10.3390/cimb44080239

T. Omanovicć Kolarić, et al., "Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis", Current Issues in Molecular Biology, vol. 44, br. 8, str. 3465-3480, Kolovoz 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:850995. [Citirano: 23.06.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:850995
Podaci o radu
Naslov (engleski)Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis
AutorTea Omanovicć Kolarić
AutorTomislav Kizivat
AutorVjera Mihaljević
AutorMilorad Zjalić
AutorInes Bilić Ćurčić
AutorLucija Kuna
AutorRobert Smolić
AutorAleksandar Včev
AutorGeorge Y. Wu
AutorMartina Smolić
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
(1) Background: With the aging of the population and polypharmacy encountered in the elderly, drug-induced steatosis (DIS) has become frequent cause of non-alcoholic steatosis (NAS). Indeed, NAS and DIS may co-exist, making the ability to distinguish between the entities ever more important. The aim of our study was to study cell culture models of NAS and DIS and determine the effects of liraglutide (LIRA) in those models. (2) Methods: Huh7 cells were treated with oleic acid (OA), or amiodarone (AMD) to establish models of NAS and DIS, respectively. Cells were treated with LIRA and cell viability was assessed by MTT, lipid accumulation by Oil-Red-O staining and triglyceride assay, and intracellular signals involved in hepatosteatosis were quantitated by RT-PCR. (3) Results: After exposure to various OA and AMD concentrations, those that achieved 80% of cells viabilities were used in further experiments to establish NAS and DIS models using 0.5 mM OA and 20 µM AMD, respectively. In both models, LIRA increased cell viability (p < 0.01). Lipid accumulation was increased in both models, with microsteatotic pattern in DIS, and macrosteatotic pattern in NAS which corresponds to greater triglyceride accumulation in latter. LIRA ameliorated these changes (p < 0.001), and downregulated expression of lipogenic ACSL1, PPARγ, and SREBP-1c pathways in the liver (p < 0.01) (4) Conclusions: LIRA ameliorates hepatocyte steatosis in Huh7 cell culture models of NAS and DIS.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCurrent Issues in Molecular Biology
Brojčani podacivol. 44, br. 8, str. 3465-3480
e-ISSN1467-3045
DOIhttps://doi.org/10.3390/cimb44080239
URN:NBNurn:nbn:hr:184:850995
Datum objave publikacije2022-08-02
URL dokumentahttps://www.mdpi.com/1467-3045/44/8/239
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-08-12 11:24:39