prikaz prve stranice dokumenta Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro
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Scientific paper - Original scientific paper
Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro
Journal of Reproductive Immunology, 95 (2012), 1-2; 36-45. https://doi.org/10.1016/j.jri.2012.06.002
Laškarin, Gordana; Medančić, Suzana Sršen; Redžovic, Arnela; Durić, Danijel; Rukavina, Daniel

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

Cite this document

Laškarin, G., Medančić, S. S., Redžovic, A., Durić, D. & Rukavina, D. (2012). Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro. Journal of Reproductive Immunology, 95. (1-2), 36-45. doi: 10.1016/j.jri.2012.06.002

Laškarin, Gordana, et al. "Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro." Journal of Reproductive Immunology, vol. 95, no. 1-2, 2012, pp. 36-45. https://doi.org/10.1016/j.jri.2012.06.002

Laškarin, Gordana, Suzana Sršen Medančić, Arnela Redžovic, Danijel Durić and Daniel Rukavina. "Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro." Journal of Reproductive Immunology 95, no. 1-2 (2012): 36-45. https://doi.org/10.1016/j.jri.2012.06.002

Laškarin, G., et al. (2012) 'Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro', Journal of Reproductive Immunology, 95(1-2), pp. 36-45. doi: 10.1016/j.jri.2012.06.002

Laškarin G, Medančić SS, Redžovic A, Durić D, Rukavina D. Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro. Journal of Reproductive Immunology [Internet]. 2012 [cited 2024 December 28];95(1-2):36-45. doi: 10.1016/j.jri.2012.06.002

G. Laškarin, S. S. Medančić, A. Redžovic, D. Durić and D. Rukavina, "Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro", Journal of Reproductive Immunology, vol. 95, no. 1-2, pp. 36-45, 2012. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:420822. [Accessed: 28 December 2024]

Metadata
Title (english)Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro
AuthorGordana Laškarin
AuthorSuzana Sršen Medančić
AuthorArnela Redžovic
AuthorDanijel Durić
AuthorDaniel Rukavina
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Surgery)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Oncology and Radiotherapy)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Abstract (english)
Mucin 1 (MUC1) forms a glycocalyx on the surface of decidual epithelial cells that needs to be removed for successful embryo attachment. We investigated whether MUC1 affects human early pregnancy decidual CD14+ cells and their interactions with cognate decidual natural killer (NK) cells. FITC-dextran internalisation, surface and intracellular antigen levels, and proliferation of CD14+ and/or CD56+ cells were analysed by flow cytometry. Magnetic separation was used to purify CD56+ and CD14+ cells. Uncultured CD14+ cells expressed a negligible percentage of CD1a and CD83 molecules. They expressed lower levels of CD16, and higher levels of endocytic mannose receptors (MR), dendritic cell-specific intercellular adhesion molecule grabbing non-integrin (DC-SIGN), proinflammatory chemokine CC receptor 5 (CCR5), and CD163 receptor, than their peripheral blood counterparts. Lipopolysaccharide stimulation did not affect FITC-dextran internalisation in CD14+ cells. MUC1 bound and internalised, in a dose-dependent manner, the carbohydrate recognition domain of MR, increasing the decoy IL-1 receptor type II and decreasing IL-15 expression in CD14+ cells. In the presence of MUC1-treated macrophages, the expression levels of the proliferation and cytotoxic mediators (perforin, Fas ligand and TNF-related activation-induced ligand or TRAIL) was attenuated, while that of the anti-inflammatory chemokine CCL17 was increased, in NK cells compared with untreated macrophages. In conclusion, MUC1 supports the alternative activation of tissue-specific CD14+ cells, and may restrict proliferation of NK cells and regulate their content of cytotoxic mediators. Based on the experiments with first-trimester decidual cells in vitro, we conclude that removing MUC1 from decidual tissue might help control trophoblast invasion by NK cells.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Reproductive Immunology
Numberingvol. 95, no. 1-2, pp. 36-45
p-ISSN0165-0378
DOIhttps://doi.org/10.1016/j.jri.2012.06.002
URN:NBNurn:nbn:hr:184:420822
Publication2012
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S0165037812005864
Type of resourceText
Access conditionsRestricted access
Terms of useLicence In copyright icon
Created on2023-06-30 08:18:40