prikaz prve stranice dokumenta Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro
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Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro
Journal of Reproductive Immunology, 95 (2012), 1-2; 36-45. https://doi.org/10.1016/j.jri.2012.06.002
Laškarin, Gordana; Medančić, Suzana Sršen; Redžovic, Arnela; Durić, Danijel; Rukavina, Daniel

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

Citirajte ovaj rad

Laškarin, G., Medančić, S. S., Redžovic, A., Durić, D. i Rukavina, D. (2012). Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro. Journal of Reproductive Immunology, 95. (1-2), 36-45. doi: 10.1016/j.jri.2012.06.002

Laškarin, Gordana, et al. "Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro." Journal of Reproductive Immunology, vol. 95, br. 1-2, 2012, str. 36-45. https://doi.org/10.1016/j.jri.2012.06.002

Laškarin, Gordana, Suzana Sršen Medančić, Arnela Redžovic, Danijel Durić i Daniel Rukavina. "Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro." Journal of Reproductive Immunology 95, br. 1-2 (2012): 36-45. https://doi.org/10.1016/j.jri.2012.06.002

Laškarin, G., et al. (2012) 'Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro', Journal of Reproductive Immunology, 95(1-2), str. 36-45. doi: 10.1016/j.jri.2012.06.002

Laškarin G, Medančić SS, Redžovic A, Durić D, Rukavina D. Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro. Journal of Reproductive Immunology [Internet]. 2012. [pristupljeno 29.12.2024.];95(1-2):36-45. doi: 10.1016/j.jri.2012.06.002

G. Laškarin, S. S. Medančić, A. Redžovic, D. Durić i D. Rukavina, "Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro", Journal of Reproductive Immunology, vol. 95, br. 1-2, str. 36-45, 2012. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:420822. [Citirano: 29.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:420822
Podaci o radu
Naslov (engleski)Specific decidual CD14+ cells hamper cognate NK cell proliferation and cytolytic mediator expression after mucin 1 treatment in vitro
AutorGordana Laškarin
AutorSuzana Sršen Medančić
AutorArnela Redžovic
AutorDanijel Durić
AutorDaniel Rukavina
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za kirurgiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za onkologiju i radioterapiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Imunologija
Sažetak (engleski)
Mucin 1 (MUC1) forms a glycocalyx on the surface of decidual epithelial cells that needs to be removed for successful embryo attachment. We investigated whether MUC1 affects human early pregnancy decidual CD14+ cells and their interactions with cognate decidual natural killer (NK) cells. FITC-dextran internalisation, surface and intracellular antigen levels, and proliferation of CD14+ and/or CD56+ cells were analysed by flow cytometry. Magnetic separation was used to purify CD56+ and CD14+ cells. Uncultured CD14+ cells expressed a negligible percentage of CD1a and CD83 molecules. They expressed lower levels of CD16, and higher levels of endocytic mannose receptors (MR), dendritic cell-specific intercellular adhesion molecule grabbing non-integrin (DC-SIGN), proinflammatory chemokine CC receptor 5 (CCR5), and CD163 receptor, than their peripheral blood counterparts. Lipopolysaccharide stimulation did not affect FITC-dextran internalisation in CD14+ cells. MUC1 bound and internalised, in a dose-dependent manner, the carbohydrate recognition domain of MR, increasing the decoy IL-1 receptor type II and decreasing IL-15 expression in CD14+ cells. In the presence of MUC1-treated macrophages, the expression levels of the proliferation and cytotoxic mediators (perforin, Fas ligand and TNF-related activation-induced ligand or TRAIL) was attenuated, while that of the anti-inflammatory chemokine CCL17 was increased, in NK cells compared with untreated macrophages. In conclusion, MUC1 supports the alternative activation of tissue-specific CD14+ cells, and may restrict proliferation of NK cells and regulate their content of cytotoxic mediators. Based on the experiments with first-trimester decidual cells in vitro, we conclude that removing MUC1 from decidual tissue might help control trophoblast invasion by NK cells.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of Reproductive Immunology
Brojčani podacivol. 95, br. 1-2, str. 36-45
p-ISSN0165-0378
DOIhttps://doi.org/10.1016/j.jri.2012.06.002
URN:NBNurn:nbn:hr:184:420822
Datum objave publikacije2012
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S0165037812005864
Vrsta resursaTekst
Prava pristupaOgraničen pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2023-06-30 08:18:40