prikaz prve stranice dokumenta Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface
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Scientific paper - Original scientific paper
Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface
Journal of Reproductive Immunology, 97 (2013), 2; 186-196. https://doi.org/10.1016/j.jri.2012.11.003
Veljković Vujaklija, Danijela; Dominović, Marin; Gulić, Tamara; Mahmutefendic, Hana; Haller, Herman; Saito, Shigeru; Rukavina, Daniel

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Veljković Vujaklija, D., Dominović, M., Gulić, T., Mahmutefendic, H., Haller, H., Saito, S. & Rukavina, D. (2013). Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface. Journal of Reproductive Immunology, 97. (2), 186-196. doi: 10.1016/j.jri.2012.11.003

Veljković Vujaklija, Danijela, et al. "Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface." Journal of Reproductive Immunology, vol. 97, no. 2, 2013, pp. 186-196. https://doi.org/10.1016/j.jri.2012.11.003

Veljković Vujaklija, Danijela, Marin Dominović, Tamara Gulić, Hana Mahmutefendic, Herman Haller, Shigeru Saito and Daniel Rukavina. "Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface." Journal of Reproductive Immunology 97, no. 2 (2013): 186-196. https://doi.org/10.1016/j.jri.2012.11.003

Veljković Vujaklija, D., et al. (2013) 'Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface', Journal of Reproductive Immunology, 97(2), pp. 186-196. doi: 10.1016/j.jri.2012.11.003

Veljković Vujaklija D, Dominović M, Gulić T, Mahmutefendic H, Haller H, Saito S, and sur.. Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface. Journal of Reproductive Immunology [Internet]. 2013 April [cited 2024 April 30];97(2):186-196. doi: 10.1016/j.jri.2012.11.003

D. Veljković Vujaklija, et al., "Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface", Journal of Reproductive Immunology, vol. 97, no. 2, pp. 186-196, April 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:825059. [Accessed: 30 April 2024]

Metadata
Title (english)Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface
AuthorDanijela Veljković Vujaklija
AuthorMarin Dominović
AuthorTamara Gulić
AuthorHana Mahmutefendic
AuthorHerman Haller
AuthorShigeru Saito
AuthorDaniel Rukavina
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Physiology, Immunology and Pathophxsiology)
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Department of Gynaecology and Obstetrics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Immunology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Gynecology and Obstetrics
Abstract (english)
Granulysin (GNLY) is a cytolytic/apoptotic molecule highly expressed in immune cells, particularly NK cells, at the maternal-fetal interface. The primary function of GNLY is to carry out lysis or apoptosis induction in target cells, tumor cells or cells infected by intracellular pathogens. To exert some of its functions GNLY needs to collaborate with perforin. The purpose of this study was to determine: (a) the expression of GNLY at the gene and protein levels at the maternal-fetal interface, (b) the relationship(s) between GNLY and perforin, and (c) GNLY secretion by NK cells stimulated by the NK-sensitive K562 cell line and its HLA-C and HLA-G transfectants. GNLY and perforin genes were found to be highly activated at the interface. GNLY mRNA was present at significantly higher levels compared with other cytolytic/apoptotic molecules. Confocal microscopy analysis showed that most first trimester pregnancy decidual lymphocytes simultaneously contained both GNLY and perforin protein in their cytoplasm, with a punctuate pattern consistent with granule localization. In contrast to peripheral blood, in unstimulated decidual lymphocytes GNLY and perforin rarely co-localized (10% of GNLY-positive cells and 20% of perforin-positive cells were positive for both proteins). Contact between decidual lymphocytes and K562 cells caused GNLY and perforin to be expressed in the same granules (approximately 50% co-localization), i.e., to attain the pattern seen in peripheral blood lymphocytes. The abundant GNLY secretion by decidual NK cells compared with peripheral blood NK cells after 2. h of contact with the NK-sensitive K562 cells and K562 transfectants was striking.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleJournal of Reproductive Immunology
Numberingvol. 97, no. 2, pp. 186-196
p-ISSN0165-0378
DOIhttps://doi.org/10.1016/j.jri.2012.11.003
URN:NBNurn:nbn:hr:184:825059
Publication2013-04
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S0165037813000028
Type of resourceText
Access conditionsInstitutional access
Terms of useLicence In copyright icon
Created on2023-09-18 10:34:59