prikaz prve stranice dokumenta Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface
Pristup korisnicima matične ustanove
Znanstveni rad - Izvorni znanstveni rad
Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface
Journal of Reproductive Immunology, 97 (2013), 2; 186-196. https://doi.org/10.1016/j.jri.2012.11.003
Veljković Vujaklija, Danijela; Dominović, Marin; Gulić, Tamara; Mahmutefendic, Hana; Haller, Herman; Saito, Shigeru; Rukavina, Daniel

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

Citirajte ovaj rad

Veljković Vujaklija, D., Dominović, M., Gulić, T., Mahmutefendic, H., Haller, H., Saito, S. i Rukavina, D. (2013). Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface. Journal of Reproductive Immunology, 97. (2), 186-196. doi: 10.1016/j.jri.2012.11.003

Veljković Vujaklija, Danijela, et al. "Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface." Journal of Reproductive Immunology, vol. 97, br. 2, 2013, str. 186-196. https://doi.org/10.1016/j.jri.2012.11.003

Veljković Vujaklija, Danijela, Marin Dominović, Tamara Gulić, Hana Mahmutefendic, Herman Haller, Shigeru Saito i Daniel Rukavina. "Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface." Journal of Reproductive Immunology 97, br. 2 (2013): 186-196. https://doi.org/10.1016/j.jri.2012.11.003

Veljković Vujaklija, D., et al. (2013) 'Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface', Journal of Reproductive Immunology, 97(2), str. 186-196. doi: 10.1016/j.jri.2012.11.003

Veljković Vujaklija D, Dominović M, Gulić T, Mahmutefendic H, Haller H, Saito S, i sur.. Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface. Journal of Reproductive Immunology [Internet]. 04.2013. [pristupljeno 23.11.2024.];97(2):186-196. doi: 10.1016/j.jri.2012.11.003

D. Veljković Vujaklija, et al., "Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface", Journal of Reproductive Immunology, vol. 97, br. 2, str. 186-196, Travanj 2013. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:825059. [Citirano: 23.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:825059
Podaci o radu
Naslov (engleski)Granulysin expression and the interplay of granulysin and perforin at the maternal–fetal interface
AutorDanijela Veljković Vujaklija
AutorMarin Dominović
AutorTamara Gulić
AutorHana Mahmutefendic
AutorHerman Haller
AutorShigeru Saito
AutorDaniel Rukavina
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za ginekologiju i opstetriciju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Imunologija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Ginekologija i opstetricija
Sažetak (engleski)
Granulysin (GNLY) is a cytolytic/apoptotic molecule highly expressed in immune cells, particularly NK cells, at the maternal-fetal interface. The primary function of GNLY is to carry out lysis or apoptosis induction in target cells, tumor cells or cells infected by intracellular pathogens. To exert some of its functions GNLY needs to collaborate with perforin. The purpose of this study was to determine: (a) the expression of GNLY at the gene and protein levels at the maternal-fetal interface, (b) the relationship(s) between GNLY and perforin, and (c) GNLY secretion by NK cells stimulated by the NK-sensitive K562 cell line and its HLA-C and HLA-G transfectants. GNLY and perforin genes were found to be highly activated at the interface. GNLY mRNA was present at significantly higher levels compared with other cytolytic/apoptotic molecules. Confocal microscopy analysis showed that most first trimester pregnancy decidual lymphocytes simultaneously contained both GNLY and perforin protein in their cytoplasm, with a punctuate pattern consistent with granule localization. In contrast to peripheral blood, in unstimulated decidual lymphocytes GNLY and perforin rarely co-localized (10% of GNLY-positive cells and 20% of perforin-positive cells were positive for both proteins). Contact between decidual lymphocytes and K562 cells caused GNLY and perforin to be expressed in the same granules (approximately 50% co-localization), i.e., to attain the pattern seen in peripheral blood lymphocytes. The abundant GNLY secretion by decidual NK cells compared with peripheral blood NK cells after 2. h of contact with the NK-sensitive K562 cells and K562 transfectants was striking.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of Reproductive Immunology
Brojčani podacivol. 97, br. 2, str. 186-196
p-ISSN0165-0378
DOIhttps://doi.org/10.1016/j.jri.2012.11.003
URN:NBNurn:nbn:hr:184:825059
Datum objave publikacije2013-04
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S0165037813000028
Vrsta resursaTekst
Prava pristupaInstitucijski pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2023-09-18 10:34:59