prikaz prve stranice dokumenta Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile
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Scientific paper - Original scientific paper
Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile
Frontiers in Immunology, 14 (2023); 1192057. https://doi.org/10.3389/fimmu.2023.1192057
Madela-Mönchinger, Julia Cecilia; Wolf, Silver Anthony; Wyler, Emanuel; Bauer, Agnieszka; Mischke, Marius; Möller, Lars; Juranić Lisnić, Vanda; Landthaler, Markus; Malyshkina, Anna; Voigt, Sebastian

Institutional repository: Repository of the University of Rijeka, Faculty of Medicine

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Madela Mönchinger, J. C., Wolf, S. A., Wyler, E., Bauer, A., Mischke, M., Möller, L. ... Voigt, S. (2023). Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile. Frontiers in Immunology, 14.. doi: 10.3389/fimmu.2023.1192057

Madela Mönchinger, Julia Cecilia, et al. "Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile." Frontiers in Immunology, vol. 14, 2023. https://doi.org/10.3389/fimmu.2023.1192057

Madela Mönchinger, Julia Cecilia, Silver Anthony Wolf, Emanuel Wyler, Agnieszka Bauer, Marius Mischke, Lars Möller, Vanda Juranić Lisnić, Markus Landthaler, Anna Malyshkina and Sebastian Voigt. "Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile." Frontiers in Immunology 14 (2023). https://doi.org/10.3389/fimmu.2023.1192057

Madela Mönchinger, J. C., et al. (2023) 'Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile', Frontiers in Immunology, 14. doi: 10.3389/fimmu.2023.1192057

Madela Mönchinger JC, Wolf SA, Wyler E, Bauer A, Mischke M, Möller L, and sur.. Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile. Frontiers in Immunology [Internet]. 2023 November 23 [cited 2024 July 13];14. doi: 10.3389/fimmu.2023.1192057

J. C. Madela Mönchinger, et al., "Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile", Frontiers in Immunology, vol. 14, November 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:184:210736. [Accessed: 13 July 2024]

Metadata
Title (english)Rat cytomegalovirus efficiently replicates in dendritic cells and induces changes in their transcriptional profile
AuthorJulia Cecilia Madela-Mönchinger
AuthorSilver Anthony Wolf
AuthorEmanuel Wyler
AuthorAgnieszka Bauer
AuthorMarius Mischke
AuthorLars Möller
AuthorVanda Juranić Lisnić
AuthorMarkus Landthaler
AuthorAnna Malyshkina
AuthorSebastian Voigt
Author's institutionUniversity of Rijeka
Faculty of Medicine
(Center for Proteomics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Dendritic cells (DC) play a crucial role in generating and maintaining antiviral immunity. While DC are implicated in the antiviral defense by inducing T cell responses, they can also become infected by Cytomegalovirus (CMV). CMV is not only highly species-specific but also specialized in evading immune protection, and this specialization is in part due to characteristic genes encoded by a given virus. Here, we investigated whether rat CMV can infect XCR1+ DC and if infection of DC alters expression of cell surface markers and migration behavior. We demonstrate that wild-type RCMV and a mutant virus lacking the γ-chemokine ligand xcl1 (Δvxcl1 RCMV) infect splenic rat DC ex vivo and identify viral assembly compartments. Replication-competent RCMV reduced XCR1 and MHCII surface expression. Further, gene expression of infected DC was analyzed by bulk RNA-sequencing (RNA-Seq). RCMV infection reverted a state of DC activation that was induced by DC cultivation. On the functional level, we observed impaired chemotactic activity of infected XCR1+ DC compared to mock-treated cells. We therefore speculate that as a result of RCMV infection, DC exhibit diminished XCR1 expression and are thereby blocked from the lymphocyte crosstalk.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleFrontiers in Immunology
Numberingvol. 14, 1192057
e-ISSN1664-3224
DOIhttps://doi.org/10.3389/fimmu.2023.1192057
URN:NBNurn:nbn:hr:184:210736
Publication2023-11-23
Document URLhttps://www.frontiersin.org/articles/10.3389/fimmu.2023.1192057/full
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-12-21 07:57:13