prikaz prve stranice dokumenta Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis
  Download
PDF 4.64 MB
Scientific paper - Original scientific paper
Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis
Cells, 8 (2019), 9; 1001. https://doi.org/10.3390/cells8091001
Milković, Lidija; Tomljanović, Marko; Čipak Gašparović, Ana; Novak Kujundžić, Renata; Šimunić, Dina; Konjevoda, Paško; Mojzeš, Anamarija; Đaković, Nikola; Žarković, Neven; Gall Trošelj, Koraljka

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Milković, L., Tomljanović, M., Čipak Gašparović, A., Novak Kujundžić, R., Šimunić, D., Konjevoda, P. ... Gall Trošelj, K. (2019). Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis. Cells, 8. (9). doi: 10.3390/cells8091001

Milković, Lidija, et al. "Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis." Cells, vol. 8, no. 9, 2019. https://doi.org/10.3390/cells8091001

Milković, Lidija, Marko Tomljanović, Ana Čipak Gašparović, Renata Novak Kujundžić, Dina Šimunić, Paško Konjevoda, Anamarija Mojzeš, Nikola Đaković, Neven Žarković and Koraljka Gall Trošelj. "Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis." Cells 8, no. 9 (2019). https://doi.org/10.3390/cells8091001

Milković, L., et al. (2019) 'Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis', Cells, 8(9). doi: 10.3390/cells8091001

Milković L, Tomljanović M, Čipak Gašparović A, Novak Kujundžić R, Šimunić D, Konjevoda P, and sur.. Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis. Cells [Internet]. 2019 August 29 [cited 2024 November 26];8(9). doi: 10.3390/cells8091001

L. Milković, et al., "Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis", Cells, vol. 8, no. 9, August 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:192724. [Accessed: 26 November 2024]

Metadata
Title (english)Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis
AuthorLidija Milković
AuthorMarko Tomljanović
AuthorAna Čipak Gašparović
AuthorRenata Novak Kujundžić
AuthorDina Šimunić
AuthorPaško Konjevoda
AuthorAnamarija Mojzeš
AuthorNikola Đaković
AuthorNeven Žarković
AuthorKoraljka Gall Trošelj
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Clinical Oncology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Oncology
Abstract (english)
Nutritional stress disturbs the cellular redox-status, which is characterized by the increased generation of reactive oxygen species (ROS). The NRF2-NQO1 axis represents a protective mechanism against ROS. Its strength is cell type-specific. FaDu, Cal 27 and Detroit 562 cells differ with respect to basal NQO1 activity. These cells were grown for 48 hours in nutritional conditions (NC): (a) Low glucose–NC2, (b) no glucose, no glutamine–NC3, (c) no glucose with glutamine–NC4. After determining the viability, proliferation and ROS generation, NC2 and NC3 were chosen for further exploration. These conditions were also applied to IMR-90 fibroblasts. The transcripts/transcript variants of NRF2 and NQO1 were quantified and transcript variants were characterized. The proteins (NRF2, NQO1 and TP53) were analyzed by a western blot in both cellular fractions. Under NC2, the NRF2-NQO1 axis did not appear activated in the cancer cell lines. Under NC3, the NRF2-NQO1axis appeared slightly activated in Detroit 562. There are opposite trends with respect to TP53 nuclear signal when comparing Cal 27 and Detroit 562 to FaDu, under NC2 and NC3. The strong activation of the NRF2-NQO1 axis in IMR-90 resulted in an increased expression of catalytically deficient NQO1, due to NQO1*2/*2 polymorphism (rs1800566). The presented results call for a comprehensive exploration of the stress response in complex biological systems.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCells
Numberingvol. 8, no. 9, 1001
e-ISSN2073-4409
DOIhttps://doi.org/10.3390/cells8091001
URN:NBNurn:nbn:hr:105:192724
Publication2019-08-29
Document URLhttps://www.mdpi.com/2073-4409/8/9/1001
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2021-03-10 10:23:43