prikaz prve stranice dokumenta Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis
  Preuzmi
PDF 4.64 MB
Znanstveni rad - Izvorni znanstveni rad
Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis
Cells, 8 (2019), 9; 1001. https://doi.org/10.3390/cells8091001
Milković, Lidija; Tomljanović, Marko; Čipak Gašparović, Ana; Novak Kujundžić, Renata; Šimunić, Dina; Konjevoda, Paško; Mojzeš, Anamarija; Đaković, Nikola; Žarković, Neven; Gall Trošelj, Koraljka

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Citirajte ovaj rad

Milković, L., Tomljanović, M., Čipak Gašparović, A., Novak Kujundžić, R., Šimunić, D., Konjevoda, P. ... Gall Trošelj, K. (2019). Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis. Cells, 8. (9). doi: 10.3390/cells8091001

Milković, Lidija, et al. "Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis." Cells, vol. 8, br. 9, 2019. https://doi.org/10.3390/cells8091001

Milković, Lidija, Marko Tomljanović, Ana Čipak Gašparović, Renata Novak Kujundžić, Dina Šimunić, Paško Konjevoda, Anamarija Mojzeš, Nikola Đaković, Neven Žarković i Koraljka Gall Trošelj. "Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis." Cells 8, br. 9 (2019). https://doi.org/10.3390/cells8091001

Milković, L., et al. (2019) 'Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis', Cells, 8(9). doi: 10.3390/cells8091001

Milković L, Tomljanović M, Čipak Gašparović A, Novak Kujundžić R, Šimunić D, Konjevoda P, i sur.. Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis. Cells [Internet]. 29.08.2019. [pristupljeno 28.12.2024.];8(9). doi: 10.3390/cells8091001

L. Milković, et al., "Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis", Cells, vol. 8, br. 9, Kolovoz 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:192724. [Citirano: 28.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:192724
Podaci o radu
Naslov (engleski)Nutritional Stress in Head and Neck Cancer Originating Cell Lines: The Sensitivity of the NRF2-NQO1 Axis
AutorLidija Milković
AutorMarko Tomljanović
AutorAna Čipak Gašparović
AutorRenata Novak Kujundžić
AutorDina Šimunić
AutorPaško Konjevoda
AutorAnamarija Mojzeš
AutorNikola Đaković
AutorNeven Žarković
AutorKoraljka Gall Trošelj
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA KLINIČKU ONKOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Onkologija
Sažetak (engleski)
Nutritional stress disturbs the cellular redox-status, which is characterized by the increased generation of reactive oxygen species (ROS). The NRF2-NQO1 axis represents a protective mechanism against ROS. Its strength is cell type-specific. FaDu, Cal 27 and Detroit 562 cells differ with respect to basal NQO1 activity. These cells were grown for 48 hours in nutritional conditions (NC): (a) Low glucose–NC2, (b) no glucose, no glutamine–NC3, (c) no glucose with glutamine–NC4. After determining the viability, proliferation and ROS generation, NC2 and NC3 were chosen for further exploration. These conditions were also applied to IMR-90 fibroblasts. The transcripts/transcript variants of NRF2 and NQO1 were quantified and transcript variants were characterized. The proteins (NRF2, NQO1 and TP53) were analyzed by a western blot in both cellular fractions. Under NC2, the NRF2-NQO1 axis did not appear activated in the cancer cell lines. Under NC3, the NRF2-NQO1axis appeared slightly activated in Detroit 562. There are opposite trends with respect to TP53 nuclear signal when comparing Cal 27 and Detroit 562 to FaDu, under NC2 and NC3. The strong activation of the NRF2-NQO1 axis in IMR-90 resulted in an increased expression of catalytically deficient NQO1, due to NQO1*2/*2 polymorphism (rs1800566). The presented results call for a comprehensive exploration of the stress response in complex biological systems.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCells
Brojčani podacivol. 8, br. 9, 1001
e-ISSN2073-4409
DOIhttps://doi.org/10.3390/cells8091001
URN:NBNurn:nbn:hr:105:192724
Datum objave publikacije2019-08-29
URL dokumentahttps://www.mdpi.com/2073-4409/8/9/1001
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2021-03-10 10:23:43