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Scientific paper - Original scientific paper
The role of magnetic resonance imaging and the expression of MMP-9 protein in the analysis of carotid atherosclerotic plaques in patients undergoing carotid endarterectomy: a prospective pilot study
Reviews in Cardiovascular Medicine, 22 (2021), 4; 1611-1620. https://doi.org/10.31083/j.rcm2204167
Sef, Davorin; Milošević, Milan; Oštrić, Marin; Meštrović, Tomislav; Jernej, Bojan; Kovačić, Slavica; Kovačević, Miljenko; Škrtić, Anita; Vidjak, Vinko

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Sef, D., Milošević, M., Oštrić, M., Meštrović, T., Jernej, B., Kovačić, S. ... Vidjak, V. (2021). The role of magnetic resonance imaging and the expression of MMP-9 protein in the analysis of carotid atherosclerotic plaques in patients undergoing carotid endarterectomy: a prospective pilot study. Reviews in Cardiovascular Medicine, 22. (4), 1611-1620. doi: 10.31083/j.rcm2204167

Sef, Davorin, et al. "The role of magnetic resonance imaging and the expression of MMP-9 protein in the analysis of carotid atherosclerotic plaques in patients undergoing carotid endarterectomy: a prospective pilot study." Reviews in Cardiovascular Medicine, vol. 22, no. 4, 2021, pp. 1611-1620. https://doi.org/10.31083/j.rcm2204167

Sef, Davorin, Milan Milošević, Marin Oštrić, Tomislav Meštrović, Bojan Jernej, Slavica Kovačić, Miljenko Kovačević, Anita Škrtić and Vinko Vidjak. "The role of magnetic resonance imaging and the expression of MMP-9 protein in the analysis of carotid atherosclerotic plaques in patients undergoing carotid endarterectomy: a prospective pilot study." Reviews in Cardiovascular Medicine 22, no. 4 (2021): 1611-1620. https://doi.org/10.31083/j.rcm2204167

Sef, D., et al. (2021) 'The role of magnetic resonance imaging and the expression of MMP-9 protein in the analysis of carotid atherosclerotic plaques in patients undergoing carotid endarterectomy: a prospective pilot study', Reviews in Cardiovascular Medicine, 22(4), pp. 1611-1620. doi: 10.31083/j.rcm2204167

Sef D, Milošević M, Oštrić M, Meštrović T, Jernej B, Kovačić S, and sur.. The role of magnetic resonance imaging and the expression of MMP-9 protein in the analysis of carotid atherosclerotic plaques in patients undergoing carotid endarterectomy: a prospective pilot study. Reviews in Cardiovascular Medicine [Internet]. 2021 December 22 [cited 2024 November 22];22(4):1611-1620. doi: 10.31083/j.rcm2204167

D. Sef, et al., "The role of magnetic resonance imaging and the expression of MMP-9 protein in the analysis of carotid atherosclerotic plaques in patients undergoing carotid endarterectomy: a prospective pilot study", Reviews in Cardiovascular Medicine, vol. 22, no. 4, pp. 1611-1620, December 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:929089. [Accessed: 22 November 2024]

Metadata
Title (english)The role of magnetic resonance imaging and the expression of MMP-9 protein in the analysis of carotid atherosclerotic plaques in patients undergoing carotid endarterectomy: a prospective pilot study
AuthorDavorin Sef
AuthorMilan Milošević
AuthorMarin Oštrić
AuthorTomislav Meštrović
AuthorBojan Jernej
AuthorSlavica Kovačić
AuthorMiljenko Kovačević
AuthorAnita Škrtić
AuthorVinko Vidjak
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Surgery)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Radiology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Environmental and Occupational Health)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Radiology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Public Health and Health Care
Occupational and Sports Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Surgery
Abstract (english)
Components of carotid atherosclerotic plaque can be analysed preoperatively by non-invasive advanced imaging modalities such as magnetic resonance imaging (MRI). The expression of matrix metalloproteinase-9 protein (MMP-9), which has a potential role in remodelling of atherosclerotic plaques, can be analysed immunohistochemically. The aim of the present prospective pilot study is to analyse histological characteristics and expression of MMP-9 in carotid plaques of patients undergoing carotid endarterectomy (CEA) and to investigate the correlation with preoperative clinical symptoms and MRI features. Preoperative clinical assessment, MRI imaging, postoperative histological and immunohistochemical analyses were performed. Fifteen patients with symptomatic (7/15; 47%) and asymptomatic carotid artery stenosis undergoing CEA were included. Among symptomatic patients, 5 (71%) had recent stroke and 2 (29%) had recent transient ischaemic attack with a median timing of 6 weeks (IQR: 1, 18) before the surgery. Both groups did not significantly differ in respect to preoperative characteristics. Prevalence of unstable plaque was higher in symptomatic than asymptomatic patients, although it was not significant (63% vs. 37%, p = 0.077). The expression of MMP-9 in CD68 cells within the plaque by semiquantitative analysis was found to be significantly higher in symptomatic as compared to asymptomatic patients (86% vs. 25% with the highest expression, p = 0.014). The average microvascular density was found to be higher and lipid core area larger among both symptomatic patients and unstable carotid plaque specimens, although this did not reach statistical significance (p = 0.064 and p = 0.132, p = 0.360 and p = 0.569, respectively). Our results demonstrate that MRI is reliable in classifying carotid lesions and differentiating unstable from stable plaques. We have also shown that the expression of MMP-9 is significantly higher among symptomatic patients undergoing CEA.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleReviews in Cardiovascular Medicine
Numberingvol. 22, no. 4, pp. 1611-1620
p-ISSN2153-8174
DOIhttps://doi.org/10.31083/j.rcm2204167
URN:NBNurn:nbn:hr:105:929089
Publication2021-12-22
Document URLhttps://imrpress.com/journal/RCM/22/4/10.31083/j.rcm2204167
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-02-01 10:05:31