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Scientific paper - Original scientific paper
Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury—Immunohistochemical and Biochemical Analyses
Journal of Clinical Medicine, 11 (2022), 5; 1284. https://doi.org/10.3390/jcm11051284
Bašković, Marko; Krsnik, Dajana; Himelreich Perić, Marta; Katušić Bojanac, Ana; Sinčić, Nino; Sonicki, Zdenko; Ježek, Davor

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Bašković, M., Krsnik, D., Himelreich Perić, M., Katušić Bojanac, A., Sinčić, N., Sonicki, Z. & Ježek, D. (2022). Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury—Immunohistochemical and Biochemical Analyses. Journal of Clinical Medicine, 11. (5). doi: 10.3390/jcm11051284

Bašković, Marko, et al. "Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury—Immunohistochemical and Biochemical Analyses." Journal of Clinical Medicine, vol. 11, no. 5, 2022. https://doi.org/10.3390/jcm11051284

Bašković, Marko, Dajana Krsnik, Marta Himelreich Perić, Ana Katušić Bojanac, Nino Sinčić, Zdenko Sonicki and Davor Ježek. "Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury—Immunohistochemical and Biochemical Analyses." Journal of Clinical Medicine 11, no. 5 (2022). https://doi.org/10.3390/jcm11051284

Bašković, M., et al. (2022) 'Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury—Immunohistochemical and Biochemical Analyses', Journal of Clinical Medicine, 11(5). doi: 10.3390/jcm11051284

Bašković M, Krsnik D, Himelreich Perić M, Katušić Bojanac A, Sinčić N, Sonicki Z, and sur.. Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury—Immunohistochemical and Biochemical Analyses. Journal of Clinical Medicine [Internet]. 2022 February 26 [cited 2024 November 04];11(5). doi: 10.3390/jcm11051284

M. Bašković, et al., "Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury—Immunohistochemical and Biochemical Analyses", Journal of Clinical Medicine, vol. 11, no. 5, February 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:369326. [Accessed: 04 November 2024]

Metadata
Title (english)Astaxanthin Relieves Testicular Ischemia-Reperfusion Injury—Immunohistochemical and Biochemical Analyses
AuthorMarko Bašković
AuthorDajana Krsnik
AuthorMarta Himelreich Perić
AuthorAna Katušić Bojanac
AuthorNino Sinčić
AuthorZdenko Sonicki
AuthorDavor Ježek
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Statistics, Epidemiology and Medical Informatics)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Public Health and Health Care
Epidemiology
Abstract (english)
Testicular torsion potentially leads to acute scrotum and testicle loss, and requires prompt surgical intervention to restore testicular blood flow, despite the paradoxical negative effect of reperfusion. While no drug is yet approved for this condition, antioxidants are promising candidates. This study aimed to determine astaxanthin’s (ASX), a potent antioxidant, effect on rat testicular torsion−detorsion injury. Thirty-two prepubertal male Fischer rats were divided into four groups. Group 1 underwent sham surgery. In group 2, the right testis was twisted at 720° for 90 min. After 90 min of reperfusion, the testis was removed. ASX was administered intraperitoneally at the time of detorsion (group 3) and 45 min after detorsion (group 4). Quantification of caspase-3 positive cells and oxidative stress markers detection were determined immunohistochemically, while the malondialdehyde (MDA) value, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities were determined by colorimetric assays. The number of apoptotic caspase-3 positive cells and the MDA value were lower in group 4 compared to group 2. A significant increase in the SOD and GPx activity was observed in group 4 compared to groups 2 and 3. We conclude that ASX has a favorable effect on testicular ischemia-reperfusion injury in rats.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleJournal of Clinical Medicine
Numberingvol. 11, no. 5, 1284
e-ISSN2077-0383
DOIhttps://doi.org/10.3390/jcm11051284
URN:NBNurn:nbn:hr:105:369326
Publication2022-02-26
ProjectNumber: KK.01.1.1.01.0008
Title (croatian): Znanstveni centar izvrsnosti za reproduktivnu i regenerativnu medicinu – istraživanja novih platformi i potencijala
Leader: Slobodan Vukičević
Jurisdiction: eu
Document URLhttps://www.mdpi.com/2077-0383/11/5/1284
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-10-06 08:26:21