prikaz prve stranice dokumenta WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction
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Scientific paper - Original scientific paper
WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction
Molecular Medicine Reports, 27 (2022), 2; 28. https://doi.org/10.3892/mmr.2022.12914
Šola, Ida; Karin‑Kujundžić, Valentina; Paić, Frane; Lijović, Lada; Glibo, Mislav; Šerman, Nikola; Duic, Tihana; Škrtić, Anita; Kuna, Krunoslav; Vranic, Semir; Šerman, Ljiljana

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Šola, I., Karin‑Kujundžić, V., Paić, F., Lijović, L., Glibo, M., Šerman, N. ... Šerman, Lj. (2022). WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction. Molecular Medicine Reports, 27. (2). doi: 10.3892/mmr.2022.12914

Šola, Ida, et al. "WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction." Molecular Medicine Reports, vol. 27, no. 2, 2022. https://doi.org/10.3892/mmr.2022.12914

Šola, Ida, Valentina Karin‑Kujundžić, Frane Paić, Lada Lijović, Mislav Glibo, Nikola Šerman, Tihana Duic, et al. "WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction." Molecular Medicine Reports 27, no. 2 (2022). https://doi.org/10.3892/mmr.2022.12914

Šola, I., et al. (2022) 'WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction', Molecular Medicine Reports, 27(2). doi: 10.3892/mmr.2022.12914

Šola I, Karin‑Kujundžić V, Paić F, Lijović L, Glibo M, Šerman N, and sur.. WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction. Molecular Medicine Reports [Internet]. 2022 December 13 [cited 2024 November 22];27(2). doi: 10.3892/mmr.2022.12914

I. Šola, et al., "WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction", Molecular Medicine Reports, vol. 27, no. 2, December 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:545832. [Accessed: 22 November 2024]

Metadata
Title (english)WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction
AuthorIda Šola
AuthorValentina Karin‑Kujundžić
AuthorFrane Paić
AuthorLada Lijović
AuthorMislav Glibo
AuthorNikola Šerman
AuthorTihana Duic
AuthorAnita Škrtić
AuthorKrunoslav Kuna
AuthorSemir Vranic
AuthorLjiljana Šerman
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Placental insufficiency is a common cause of intrauterine growth restriction (IUGR). It affects ~10% of pregnancies and increases fetal and neonatal morbidity and mortality. Although Wnt and Hh pathways are crucial for embryonic development and placentation, their role in the pathology of IUGR is still not sufficiently explored. The present study analyzed the expression of positive regulators of the Wnt pathway, WNT5A and β‑catenin, and the expression of the Hh pathway negative regulator suppressor of fused (SUFU). Immunohistochemical and reverse transcription‑quantitative PCR (RT‑qPCR) assays were performed on 34 IUGR and 18 placental tissue samples from physiologic singleton‑term pregnancies. Epigenetic mechanisms of SUFU gene regulation were also investigated by methylation‑specific PCR analysis of its promoter and RT‑qPCR analysis of miR‑214‑3p and miR‑378a‑5p expression. WNT5A protein expression was higher in endothelial cells of placental villi from IUGR compared with control tissues. That was also the case for β‑catenin protein expression in trophoblasts and endothelial cells and SUFU protein expression in trophoblasts from IUGR placentas. The SUFU gene promoter remained unmethylated in all tissue samples, while miR‑214‑3p and miR‑378a‑5p were downregulated in IUGR. The present results suggested altered Wnt and Hh signaling in IUGR. DNA methylation did not appear to be a mechanism of SUFU regulation in the pathogenesis of IUGR, but its expression could be regulated by miRNA targeting.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleMolecular Medicine Reports
Numberingvol. 27, no. 2, 28
p-ISSN1791-2997
e-ISSN1791-3004
DOIhttps://doi.org/10.3892/mmr.2022.12914
URN:NBNurn:nbn:hr:105:545832
Publication2022-12-13
Document URLhttp://www.spandidos-publications.com/10.3892/mmr.2022.12914
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) icon
Created on2023-05-16 11:22:32