prikaz prve stranice dokumenta WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction
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WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction
Molecular Medicine Reports, 27 (2022), 2; 28. https://doi.org/10.3892/mmr.2022.12914
Šola, Ida; Karin‑Kujundžić, Valentina; Paić, Frane; Lijović, Lada; Glibo, Mislav; Šerman, Nikola; Duic, Tihana; Škrtić, Anita; Kuna, Krunoslav; Vranic, Semir; Šerman, Ljiljana

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

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Šola, I., Karin‑Kujundžić, V., Paić, F., Lijović, L., Glibo, M., Šerman, N. ... Šerman, Lj. (2022). WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction. Molecular Medicine Reports, 27. (2). doi: 10.3892/mmr.2022.12914

Šola, Ida, et al. "WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction." Molecular Medicine Reports, vol. 27, br. 2, 2022. https://doi.org/10.3892/mmr.2022.12914

Šola, Ida, Valentina Karin‑Kujundžić, Frane Paić, Lada Lijović, Mislav Glibo, Nikola Šerman, Tihana Duic, et al. "WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction." Molecular Medicine Reports 27, br. 2 (2022). https://doi.org/10.3892/mmr.2022.12914

Šola, I., et al. (2022) 'WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction', Molecular Medicine Reports, 27(2). doi: 10.3892/mmr.2022.12914

Šola I, Karin‑Kujundžić V, Paić F, Lijović L, Glibo M, Šerman N, i sur.. WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction. Molecular Medicine Reports [Internet]. 13.12.2022. [pristupljeno 29.04.2024.];27(2). doi: 10.3892/mmr.2022.12914

I. Šola, et al., "WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction", Molecular Medicine Reports, vol. 27, br. 2, Prosinac 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:545832. [Citirano: 29.04.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:545832
Podaci o radu
Naslov (engleski)WNT5A, β‑catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction
AutorIda Šola
AutorValentina Karin‑Kujundžić
AutorFrane Paić
AutorLada Lijović
AutorMislav Glibo
AutorNikola Šerman
AutorTihana Duic
AutorAnita Škrtić
AutorKrunoslav Kuna
AutorSemir Vranic
AutorLjiljana Šerman
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU BIOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
Placental insufficiency is a common cause of intrauterine growth restriction (IUGR). It affects ~10% of pregnancies and increases fetal and neonatal morbidity and mortality. Although Wnt and Hh pathways are crucial for embryonic development and placentation, their role in the pathology of IUGR is still not sufficiently explored. The present study analyzed the expression of positive regulators of the Wnt pathway, WNT5A and β‑catenin, and the expression of the Hh pathway negative regulator suppressor of fused (SUFU). Immunohistochemical and reverse transcription‑quantitative PCR (RT‑qPCR) assays were performed on 34 IUGR and 18 placental tissue samples from physiologic singleton‑term pregnancies. Epigenetic mechanisms of SUFU gene regulation were also investigated by methylation‑specific PCR analysis of its promoter and RT‑qPCR analysis of miR‑214‑3p and miR‑378a‑5p expression. WNT5A protein expression was higher in endothelial cells of placental villi from IUGR compared with control tissues. That was also the case for β‑catenin protein expression in trophoblasts and endothelial cells and SUFU protein expression in trophoblasts from IUGR placentas. The SUFU gene promoter remained unmethylated in all tissue samples, while miR‑214‑3p and miR‑378a‑5p were downregulated in IUGR. The present results suggested altered Wnt and Hh signaling in IUGR. DNA methylation did not appear to be a mechanism of SUFU regulation in the pathogenesis of IUGR, but its expression could be regulated by miRNA targeting.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaMolecular Medicine Reports
Brojčani podacivol. 27, br. 2, 28
p-ISSN1791-2997
e-ISSN1791-3004
DOIhttps://doi.org/10.3892/mmr.2022.12914
URN:NBNurn:nbn:hr:105:545832
Datum objave publikacije2022-12-13
URL dokumentahttp://www.spandidos-publications.com/10.3892/mmr.2022.12914
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2023-05-16 11:22:32