prikaz prve stranice dokumenta Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine
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Scientific paper - Original scientific paper
Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine
Advances in Medical Sciences, 67 (2022), 2; 379-385. https://doi.org/10.1016/j.advms.2022.09.003
Skonieczna, Magdalena; Adamiec-Organisciok, Malgorzata; Hudy, Dorota; Dziedzic, Arkadiusz; Los, Laura; Skladany, Lubomir; Grgurević, Ivica; Filipec-Kanižaj, Tajana; Jagodzinski, Miczyslaw; Kukla, Michal; Nackiewicz, Joanna

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Skonieczna, M., Adamiec Organisciok, M., Hudy, D., Dziedzic, A., Los, L., Skladany, L. ... Nackiewicz, J. (2022). Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine. Advances in Medical Sciences, 67. (2), 379-385. doi: 10.1016/j.advms.2022.09.003

Skonieczna, Magdalena, et al. "Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine." Advances in Medical Sciences, vol. 67, no. 2, 2022, pp. 379-385. https://doi.org/10.1016/j.advms.2022.09.003

Skonieczna, Magdalena, Malgorzata Adamiec Organisciok, Dorota Hudy, Arkadiusz Dziedzic, Laura Los, Lubomir Skladany, Ivica Grgurević, et al. "Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine." Advances in Medical Sciences 67, no. 2 (2022): 379-385. https://doi.org/10.1016/j.advms.2022.09.003

Skonieczna, M., et al. (2022) 'Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine', Advances in Medical Sciences, 67(2), pp. 379-385. doi: 10.1016/j.advms.2022.09.003

Skonieczna M, Adamiec Organisciok M, Hudy D, Dziedzic A, Los L, Skladany L, and sur.. Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine. Advances in Medical Sciences [Internet]. 2022 September [cited 2024 December 27];67(2):379-385. doi: 10.1016/j.advms.2022.09.003

M. Skonieczna, et al., "Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine", Advances in Medical Sciences, vol. 67, no. 2, pp. 379-385, September 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:020762. [Accessed: 27 December 2024]

Metadata
Title (english)Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine
AuthorMagdalena Skonieczna
AuthorMalgorzata Adamiec-Organisciok
AuthorDorota Hudy
AuthorArkadiusz Dziedzic
AuthorLaura Los
AuthorLubomir Skladany
AuthorIvica Grgurević
AuthorTajana Filipec-Kanižaj
AuthorMiczyslaw Jagodzinski
AuthorMichal Kukla
AuthorJoanna Nackiewicz
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Internal Medicine)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Abstract (english)
Purpose: Human carcinoma cells with different p53 status exposed to a combination of bioactive substances, resveratrol and berberine, revealed different responses in cell viability via p53-dependant apoptosis pathway activation. Materials and methods: Using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, we investigated various and opposing effects in hepatocellular carcinoma cells, Hep-G2 and Hep-3B with different p53-status. Results: Cells decreased in viability after treatment with dose-dependent concentrations of resveratrol and berberine. Hep-3B p53 mutants were more sensitive in comparison to the p53 wild type Hep-G2 cell line. A synergistic effect was observed after treatment of Hep-3B cells with a combination of resveratrol/berberine ratios in favor of resveratrol (2:1, 3:1). The results suggest that an effective concentration of berberine, in the presence of resveratrol, could be decreased even to 50% (half the IC50 for berberine) in cancer treatment. Combined treatment with berberine and resveratrol, at the investigated concentrations and fractions, significantly reduces the viability of wild type p53 Hep-G2 and null p53-mutant Hep-3B cells by 20% and 40%, respectively. Conclusions: Stronger toxic effects on viability and proliferation were observed in Hep-3B cells what is consistent with the assumptions that null p53-mutants activate apoptosis canonical pathway. In conclusion, p53 status in human hepatocellular cancer cell lines modulates responses to plant-derived therapies.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleAdvances in Medical Sciences
Numberingvol. 67, no. 2, pp. 379-385
p-ISSN1896-1126
DOIhttps://doi.org/10.1016/j.advms.2022.09.003
URN:NBNurn:nbn:hr:105:020762
Publication2022-09
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S1896112622000372
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-08-24 09:23:35