prikaz prve stranice dokumenta Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine
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Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine
Advances in Medical Sciences, 67 (2022), 2; 379-385. https://doi.org/10.1016/j.advms.2022.09.003
Skonieczna, Magdalena; Adamiec-Organisciok, Malgorzata; Hudy, Dorota; Dziedzic, Arkadiusz; Los, Laura; Skladany, Lubomir; Grgurević, Ivica; Filipec-Kanižaj, Tajana; Jagodzinski, Miczyslaw; Kukla, Michal; Nackiewicz, Joanna

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

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Skonieczna, M., Adamiec Organisciok, M., Hudy, D., Dziedzic, A., Los, L., Skladany, L. ... Nackiewicz, J. (2022). Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine. Advances in Medical Sciences, 67. (2), 379-385. doi: 10.1016/j.advms.2022.09.003

Skonieczna, Magdalena, et al. "Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine." Advances in Medical Sciences, vol. 67, br. 2, 2022, str. 379-385. https://doi.org/10.1016/j.advms.2022.09.003

Skonieczna, Magdalena, Malgorzata Adamiec Organisciok, Dorota Hudy, Arkadiusz Dziedzic, Laura Los, Lubomir Skladany, Ivica Grgurević, et al. "Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine." Advances in Medical Sciences 67, br. 2 (2022): 379-385. https://doi.org/10.1016/j.advms.2022.09.003

Skonieczna, M., et al. (2022) 'Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine', Advances in Medical Sciences, 67(2), str. 379-385. doi: 10.1016/j.advms.2022.09.003

Skonieczna M, Adamiec Organisciok M, Hudy D, Dziedzic A, Los L, Skladany L, i sur.. Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine. Advances in Medical Sciences [Internet]. 09.2022. [pristupljeno 26.12.2024.];67(2):379-385. doi: 10.1016/j.advms.2022.09.003

M. Skonieczna, et al., "Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine", Advances in Medical Sciences, vol. 67, br. 2, str. 379-385, Rujan 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:020762. [Citirano: 26.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:020762
Podaci o radu
Naslov (engleski)Hepatocellular cancer cell lines, Hep-3B and Hep-G2 display the pleiotropic response to resveratrol and berberine
AutorMagdalena Skonieczna
AutorMalgorzata Adamiec-Organisciok
AutorDorota Hudy
AutorArkadiusz Dziedzic
AutorLaura Los
AutorLubomir Skladany
AutorIvica Grgurević
AutorTajana Filipec-Kanižaj
AutorMiczyslaw Jagodzinski
AutorMichal Kukla
AutorJoanna Nackiewicz
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA INTERNU MEDICINU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Interna medicina
Sažetak (engleski)
Purpose: Human carcinoma cells with different p53 status exposed to a combination of bioactive substances, resveratrol and berberine, revealed different responses in cell viability via p53-dependant apoptosis pathway activation. Materials and methods: Using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, we investigated various and opposing effects in hepatocellular carcinoma cells, Hep-G2 and Hep-3B with different p53-status. Results: Cells decreased in viability after treatment with dose-dependent concentrations of resveratrol and berberine. Hep-3B p53 mutants were more sensitive in comparison to the p53 wild type Hep-G2 cell line. A synergistic effect was observed after treatment of Hep-3B cells with a combination of resveratrol/berberine ratios in favor of resveratrol (2:1, 3:1). The results suggest that an effective concentration of berberine, in the presence of resveratrol, could be decreased even to 50% (half the IC50 for berberine) in cancer treatment. Combined treatment with berberine and resveratrol, at the investigated concentrations and fractions, significantly reduces the viability of wild type p53 Hep-G2 and null p53-mutant Hep-3B cells by 20% and 40%, respectively. Conclusions: Stronger toxic effects on viability and proliferation were observed in Hep-3B cells what is consistent with the assumptions that null p53-mutants activate apoptosis canonical pathway. In conclusion, p53 status in human hepatocellular cancer cell lines modulates responses to plant-derived therapies.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaAdvances in Medical Sciences
Brojčani podacivol. 67, br. 2, str. 379-385
p-ISSN1896-1126
DOIhttps://doi.org/10.1016/j.advms.2022.09.003
URN:NBNurn:nbn:hr:105:020762
Datum objave publikacije2022-09
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S1896112622000372
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2023-08-24 09:23:35