prikaz prve stranice dokumenta Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?
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Scientific paper - Original scientific paper
Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?
European Journal of Clinical Microbiology & Infectious Diseases, 33 (2014), 12; 2231-2236. https://doi.org/10.1007/s10096-014-2187-7
Đaković Rode, Oktavija; Jemeršić, Lorena; Brnić, Dragan; Pandak, Nenad; Mikulić, Radojka; Begovac, Josip; Vince, Adriana

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Đaković Rode, O., Jemeršić, L., Brnić, D., Pandak, N., Mikulić, R., Begovac, J. & Vince, A. (2014). Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?. European Journal of Clinical Microbiology & Infectious Diseases, 33. (12), 2231-2236. doi: 10.1007/s10096-014-2187-7

Đaković Rode, Oktavija, et al. "Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?." European Journal of Clinical Microbiology & Infectious Diseases, vol. 33, no. 12, 2014, pp. 2231-2236. https://doi.org/10.1007/s10096-014-2187-7

Đaković Rode, Oktavija, Lorena Jemeršić, Dragan Brnić, Nenad Pandak, Radojka Mikulić, Josip Begovac and Adriana Vince. "Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?." European Journal of Clinical Microbiology & Infectious Diseases 33, no. 12 (2014): 2231-2236. https://doi.org/10.1007/s10096-014-2187-7

Đaković Rode, O., et al. (2014) 'Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?', European Journal of Clinical Microbiology & Infectious Diseases, 33(12), pp. 2231-2236. doi: 10.1007/s10096-014-2187-7

Đaković Rode O, Jemeršić L, Brnić D, Pandak N, Mikulić R, Begovac J, and sur.. Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?. European Journal of Clinical Microbiology & Infectious Diseases [Internet]. 2014 December [cited 2024 November 22];33(12):2231-2236. doi: 10.1007/s10096-014-2187-7

O. Đaković Rode, et al., "Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?", European Journal of Clinical Microbiology & Infectious Diseases, vol. 33, no. 12, pp. 2231-2236, December 2014. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:284149. [Accessed: 22 November 2024]

Metadata
Title (english)Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?
AuthorOktavija Đaković Rode
AuthorLorena Jemeršić
AuthorDragan Brnić
AuthorNenad Pandak
AuthorRadojka Mikulić
AuthorJosip Begovac
AuthorAdriana Vince
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
We assessed hepatitis E virus (HEV) seroprevalence in patients with hepatic disorders as well as in human immunodeficiency virus (HIV)-infected patients and emphasised the issue of possible non-specific anti-HEV seroresponse and need for combining diagnostic methods for hepatitis E diagnosis. Over a two-year period, from March 2011 to February 2013, we determined anti-HEV immunoglobulin M (IgM) and IgG by enzyme immunoassays (EIA; Mikrogen, Germany) in 504 hepatitis patients negative for acute viral hepatitis A-C. Furthermore, 88 samples from randomly selected consecutive HIV-infected patients were also analysed. All EIA reactive samples were additionally tested by line immunoblot assays (LIA; Mikrogen, Germany). HEV nested reverse transcription polymerase chain reaction (RT-PCR) was carried out in 14 anti-HEV IgM LIA-positive patients. Anti-HEV IgM or IgG were detected in 16.9 % of patients by EIA and confirmed by LIA in 10.7 % [95 % confidence interval (CI) 8.3-13.7 %] of hepatitis patients. HEV RNA was detected in five patients. The agreement between EIA and LIA assessed by Cohen's kappa was 0.47 (95 % CI 0.55-0.75) for IgM and 0.83 (95 % CI 0.78-0.93) for IgG. Anti-HEV IgM and IgG seroprevalence in HIV-infected patients was 1.1 %, respectively. Our findings show a rather high HEV seroprevalence in patients with elevated liver enzymes in comparison to HIV-infected patients. Discordant findings by different methods stress the need to combine complementary methods and use a two-tier approach with prudent interpretation of reactive serological results for hepatitis E diagnosis.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleEuropean Journal of Clinical Microbiology & Infectious Diseases
Numberingvol. 33, no. 12, pp. 2231-2236
p-ISSN0934-9723
DOIhttps://doi.org/10.1007/s10096-014-2187-7
URN:NBNurn:nbn:hr:105:284149
Publication2014-12
Document URLhttps://doi.org/10.1007/s10096-014-2187-7
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 08:09:30